Characterization and transcriptional analysis of the promoter region of the Duffy blood group, chemokine receptor (DARC) gene in cattle
Characterization and transcriptional analysis of the promoter region of the Duffy blood group, chemokine receptor (DARC) gene in cattle
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DOI：
10.1016/j.vetimm.2009.05.016
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发表时间：
2009-12-15
影响因子：
1.8
通讯作者：
Mota, L. S. L. S.
中科院分区：
文献类型：
--
作者：
Carvalho, T. L.;Ribolla, P. E. M.;Mota, L. S. L. S.
Duffy抗原是间日疟原虫的唯一受体，间日疟原虫是顶复门的血寄生虫，是人类间日疟的病因。大多数非洲黑人个体及其后代对这种寄生虫的抗性是由于基因启动子区域的突变，这阻止了其在红细胞上的转录。关于牛巴贝斯虫病，已知牛磺酸品种比斑马纹品种更易受寄生虫感染。为了验证相同的人类抗性是否发生在牛中，分离DARC基因的5'侧翼区并在印度牛和普通牛中表征。对4个单核苷酸多态性位点进行了鉴定和基因分型（SNP 1：EF_647729.1：g.91 C > T; SNP 2：EF_647729.1：g.405C > T; SNP 3：EF_647729.1：g.433A > G和SNP 4：EF_647729.1：g.588A > G），这些位点在每个物种的99头牛（n = 198）中显示出显著的频率差异。对分离区域的表征显示存在6种推定的单倍型、14种由单倍型形成的基因型以及许多推定的转录因子结合位点。只有胸腺嘧啶存在于SNPs 1和2上，多见于B。indicus，被观察到改变了该地区的一些网站。尽管如此，通过实时PCR对牛进行的分析显示，每个物种中最常见的纯合子基因型（与所有多态性形成对比）在DARC基因转录上没有差异。因此，原则上，可以得出结论，鉴定的多态性在巴贝斯虫病抗性的改良计划中作为分子标记物是无用的。(C)2009爱思唯尔有限公司版权所有。
The Duffy antigen is the only receptor for Plasmodium vivax, a hemoparasite of the phylum Apicomplexa and the cause of vivax malaria in humans. Resistance to this parasite in the majority of black African individuals and their descendents is due to a mutation in the gene promoter region, which blocks its transcription on erythrocytes. Regarding bovine babesiosis, it is known that taurine breeds are more susceptible to parasite infection than zebuine breeds. In order to verify whether the same human resistance occurs in bovine, the 5' flanking region of the DARC gene was isolated and characterized in Bos indicus and Bos taurus. Four single nucleotide polymorphisms were identified and genotyped (SNP1: EF_647729.1:g.91 C > T; SNP2: EF_647729.1:g.405C > T; SNP3: EF_647729.1: g.433A > G and SNP4: EF_647729.1:g.588A > G), which showed significant frequency differences among 99 bovines of each species (n = 198). Characterization of the isolated region revealed the presence of 6 putative haplotypes, 14 genotypes, which are formed by haplotypes, and numerous putative transcription factor binding sites. Only the thymine presence on SNPs 1 and 2, more common in B. indicus, was observed to alter some of the sites in this region. Despite this fact, analyses through real-time PCR on bovines that present the most common homozygote genotypes of each species, which contrast for all the polymorphism, revealed no difference on the DARC gene transcription. Thus, in principle, it was concluded that the polymorphisms identified would not be useful as molecular markers in an improvement program for resistance to babesiosis. (C) 2009 Elsevier B.V. All rights reserved.