Guidestar-assisted wavefront-shaping methods for focusing light into biological tissue.
Guidestar-assisted wavefront-shaping methods for focusing light into biological tissue.
复制标题
DOI：
10.1038/nphoton.2015.140
复制
发表时间：
2015
期刊：
影响因子：
35
通讯作者：
Yang C
中科院分区：
文献类型：
--
作者：
Horstmeyer R;Ruan H;Yang C
在生物医学光学领域，光学散射传统上将组织内的成像范围限制在1毫米的深度。最近开发的一类波前整形技术现在旨在克服这一限制，并实现对超过1厘米的光的衍射限制控制。通过在光场进入散射介质之前操纵光场的空间分布，可以在组织深处产生微米级的焦斑。为了成功地在体内操作，这些波前整形技术通常需要来自生物样品内的反馈。这篇综述总结了最近开发的“指南星”机制，提供反馈组织内聚焦。导星辅助聚焦的潜在应用包括神经元的光遗传学控制、靶向光动力学治疗和深部组织成像。
In the field of biomedical optics, optical scattering has traditionally limited the range of imaging within tissue to a depth of one millimetre. A recently developed class of wavefront-shaping techniques now aims to overcome this limit and achieve diffraction-limited control of light beyond one centimetre. By manipulating the spatial profile of an optical field before it enters a scattering medium, it is possible to create a micrometre-scale focal spot deep within tissue. To successfully operate in vivo, these wavefront-shaping techniques typically require feedback from within the biological sample. This Review summarizes recently developed ‘guidestar’ mechanisms that provide feedback for intra-tissue focusing. Potential applications of guidestar-assisted focusing include optogenetic control over neurons, targeted photodynamic therapy and deep tissue imaging.