7-Aminoactinomycin D for apoptosis staining in flow cytometry
7-Aminoactinomycin D for apoptosis staining in flow cytometry
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DOI：
10.1016/j.ab.2012.07.005
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发表时间：
2012-10-01
影响因子：
2.9
通讯作者：
Weiss, Johanna
中科院分区：
文献类型：
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作者：
Zembruski, Nadine C. L.;Stache, Vanessa;Weiss, Johanna
7-氨基放线菌素D（7-AAD）是一种DNA染料，可在流式细胞术中区分活细胞、凋亡细胞和晚期凋亡/死亡细胞。已经描述了使用7-AAD的几种染色方案，但是关于7-AAD浓度对读数的影响的数据不可用。因此，我们比较了用PE-膜联蛋白V凋亡检测试剂盒和细胞死亡检测ELISA（PLUS）在淋巴细胞和CEM人白血病细胞中用1、5、10和20 μ g/ml 7-AAD染色20分钟获得的结果。结果表明，5、10和20 μ g/ml的7-AAD染色适用于流式细胞术中细胞凋亡的定量，而1 μ g/ml的7-AAD染色则不适用。(C)2012 Elsevier Inc. All rights reserved.
7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. Several staining protocols using 7-AAD have been described, but data on the influence of the 7-AAD concentration on the readout are not available. Therefore, we compared the results obtained by staining with 1, 5, 10, and 20 mu g/ml 7-AAD for 20 min with the PE-Annexin V Apoptosis Detection Kit and Cell Death Detection ELISA(PLUS) in lymphocytes and CEM human leukemia cells. The results showed that 7-AAD staining with 5, 10, and 20 mu g/ml, but not with 1 mu g/ml, is suitable for quantification of apoptosis in flow cytometry. (C) 2012 Elsevier Inc. All rights reserved.