Molecular Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli in a University Teaching Hospital.
Molecular Characteristics of Extended-Spectrum β-Lactamase-Producing Escherichia coli in a University Teaching Hospital.
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某大学教学医院产超广谱 β-内酰胺酶大肠杆菌的分子特征。
DOI：
10.1089/mdr.2014.0083
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Shirakawa T.
中科院分区：
文献类型：
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作者：
Osawa K;Shigemura K;Shimizu R;Kato A;Kusuki M;Jikimoto T;Nakamura T;Yoshida H;Arakawa S;Fujisawa M;Shirakawa T.
超广谱β-内酰胺酶（ESBLs）的流行率在全球范围内呈上升趋势。最近，大肠杆菌O 25：H4，序列型131（ST 131）的流行性克隆产生ESBL型CTX-M-15已被报道为主要问题。本研究对72株产ESBL大肠杆菌的分子特征进行了研究。大肠杆菌分离株。采用PCR和DNA测序、质粒复制子分型、系统发育分型、重复序列PCR（rep-PCR）和多位点序列分型等方法检测ESBLblaCTX-M基因和9个毒力因子基因（papC、papEF、fimH、hlyA、iutA、sfa、eaeA、bfpA和aggR）。所有菌株均为blaCTX-M阳性。CTX-M-1组22株（30.6%），其中CTX-M-15 9株（12.5%），CTX-M-2组3株（4.2%），CTX-M-9组47株（65.3%）。产CTX-M-15菌株E. colio 25：H4 ST 131来自系统发育群B2，rep-PCR模式为d。最常见的毒力因子是fimH（72株; 100%），最常见的复制子类型是IncF型（65株; 90.3%）。CTX-M-9组与存在papa和papEF [OR（95%CI）=9.22（1.32-64.7），p=0.025]或hA [OR（95%CI）=5.57（1.17-26.4），p=0.031]显著相关。因此，我们证实了CTX-M-15-producingE.大肠杆菌O25：H4 ST 131已在日本出现，并发现与CTX-M-9组有显著的毒力因子。
The prevalence of extended-spectrum β-lactamases (ESBLs) has been increasing worldwide. Recently, a pandemic clone ofEscherichia coliO25:H4, sequence type 131 (ST131), producing ESBL-type CTX-M-15 has been reported as a major problem. In this study, we investigated the molecular characteristics of 72 ESBL-producingE. coliisolates. We detected the ESBLblaCTX-Mgene and nine virulence factor genes (papC,papEF,fimH,hlyA,iutA,sfa,eaeA,bfpA, andaggR) by PCR and DNA sequencing, plasmid replicon typing, phylogenetic grouping, repetitive-sequence-based PCR (rep-PCR), and multilocus sequence typing. All strains were positive forblaCTX-M. Twenty-two (30.6%) strains in CTX-M-1 group included 9 (12.5%) of CTX-M-15, 3 (4.2%) in CTX-M-2 group, and 47 (65.3%) strains in CTX-M-9 group. The CTX-M-15-producingE. coliO25:H4 ST131 was derived from phylogenetic group B2 and rep-PCR pattern d. The most prevalent virulence factor wasfimH(72 strains; 100%) and the most common replicon type was theIncF type (65 strains; 90.3%). The CTX-M-9 group was significantly associated with the presence ofpapCandpapEF[OR (95% CI)=9.22 (1.32–64.7),p=0.025] orhlyA[OR (95% CI)=5.57 (1.17–26.4),p=0.031]. In conclusion, we confirmed that CTX-M-15-producingE. coliO25:H4 ST131 has emerged in Japan and found significant virulence factors with CTX-M-9 group.