Short Exposure to the DNA Intercalator DRAQ5 Dislocates the Transcription Machinery and Induces Cell Death
Short Exposure to the DNA Intercalator DRAQ5 Dislocates the Transcription Machinery and Induces Cell Death
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短暂暴露于 DNA 嵌入剂 DRAQ5 会扰乱转录机制并诱导细胞死亡
DOI：
10.1111/j.1751-1097.2010.00852.x
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发表时间：
2011
影响因子：
3.3
通讯作者：
L. Héliot
中科院分区：
文献类型：
--
作者：
E. Richard;S. Causse;C. Spriet;N. Fourré;D. Trinel;X. Darzacq;B. Vandenbunder;L. Héliot
荧光探针DRAQ5能快速渗透细胞并与DNA结合，可用于活体细胞核中分子动力学和相互作用的功能研究。在5 μ mDRAQ5孵育人骨肉瘤U2OS细胞后数分钟内，RNA聚合酶II及其相关调节蛋白HEXIM和细胞周期蛋白T1在细胞核中的分布严重受损，转录受到抑制。 此外，暴露于DRAQ5 30分钟诱导24小时后U2OS细胞死亡。 在类似条件下与Hoechst 33342孵育不会诱导这些效应。这些结果强调了在使用前仔细检查用嵌入荧光染料标记DNA的功能后果的重要性。
The fluorescent probe DRAQ5 which rapidly permeates cells and binds to DNA is potentially useful for functional studies of molecular dynamics and interactions in living nuclei. Within minutes after the incubation of human osteosarcoma U2OS cells with 5 μm DRAQ5, the distributions of RNA polymerase II and some of its associated regulatory proteins HEXIM and cyclin T1 in the nucleus are severely impaired, and transcription is inhibited. Furthermore, 30 min exposure to DRAQ5 induces death of U2OS cells 24 h later. Incubation with Hoechst 33342 under similar conditions does not induce these effects. These results emphasize the importance of carefully examining the functional consequences of labeling DNA with intercalating fluorescent dyes before use.