Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury.
Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury.
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再生和非再生轴突损伤时背根神经节的表观基因组分析。
DOI：
10.1007/978-1-0716-3012-9_7
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Müller F
中科院分区：
文献类型：
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作者：
Müller F
RNA测序（RNA-seq）、染色质免疫沉淀测序（ChIP-seq）和转座酶可及染色质测序（ATAC-seq）是全基因组技术，分别提供与基因表达、染色质结合位点和染色质可及性相关的信息。在这里，我们描述了RNA-seq，H3 K9 ac，H3 K27 ac和H3 K27 me 3 ChIP-seq，以及ATAC-seq在坐骨神经或背柱轴突切断后的背根神经节（DRG）中，以表征再生与非再生轴突损伤后DRG的转录和表观遗传特征。
RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), and assay for transposase-accessible chromatin sequencing (ATAC-seq) are genome-wide techniques that provide information relative to gene expression, chromatin binding sites, and chromatin accessibility, respectively. Here we describe RNA-seq, H3K9ac, H3K27ac and H3K27me3 ChIP-seq, and ATAC-seq in dorsal root ganglia (DRG) after sciatic nerve or dorsal column axotomy, to characterize the transcriptional and epigenetic signatures of DRG upon regenerative vs non-regenerative axonal lesion.