Formation of 4-hydroxynonenal adducts with cytochrome c oxidase in rats following short-term ethanol intake
Formation of 4-hydroxynonenal adducts with cytochrome c oxidase in rats following short-term ethanol intake
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DOI：
10.1002/hep.510290611
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发表时间：
1999-06-01
期刊：
影响因子：
13.5
通讯作者：
Henderson, GI
中科院分区：
文献类型：
--
作者：
Chen, JJ;Robinson, NC;Henderson, GI
本研究探讨了体内脂质过氧化产物4-羟基壬烯醛(HNE)在乙醇引起的细胞色素c氧化酶(COX)损伤中的作用。它利用了一个急性酒精暴露的动物模型，在该模型中，肝脏线粒体中的HNE水平显著增加。妊娠雌性SD大鼠从妊娠第17天开始灌胃5g/kg乙醇(4g/kg，间隔12小时)，第19天处死。对照组给予等量葡萄糖和乙醇。从母肝和胎肝分离线粒体，用分光光度法检测COX活性。与配对喂养对照组相比，酒精暴露使母鼠COX活性降低25%，胎鼠COX活性降低43%(P
This study addresses the role of the lipid peroxidation product, 4-hydroxynonenal (HNE), in ethanol-related damage of cytochrome c oxidase (COX) in vivo. It utilizes an animal model with acute ethanol exposure in which HNE levels in liver mitochondria are strikingly increased. Pregnant female Sprague-Dawley rats were administered 5 doses of ethanol (4 gm/kg, po at 12-hour intervals) beginning on day 17 of gestation and were sacrificed on day 19. Controls were pair-fed and received dextrose isocaloric to ethanol. Mitochondria were isolated from maternal and fetal livers and COX activities were measured spectrophoto-metrically. Compared with the pair-fed controls, COX activity was decreased with exposure to ethanol by 25% in maternal rats and 43% in fetal rats (P