Immunohistochemical analysis of adhesion molecules and matrix metalloproteinases in malignant CNS lymphomas: a study comparing primary CNS malignant and CNS intravascular lymphomas
Immunohistochemical analysis of adhesion molecules and matrix metalloproteinases in malignant CNS lymphomas: a study comparing primary CNS malignant and CNS intravascular lymphomas
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DOI：
10.1007/s10014-008-0232-x
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发表时间：
2008-11-01
影响因子：
3.3
通讯作者：
Yoshimine, Toshiki
中科院分区：
文献类型：
--
作者：
Kinoshita, Manabu;Izumoto, Shuichi;Yoshimine, Toshiki
两种不同形式的恶性淋巴瘤可以侵入中枢神经系统（CNS）。虽然原发性中枢神经系统恶性淋巴瘤（PCNSML）侵入脑实质，但血管内淋巴瘤（IVL）在脉管系统中形成肿瘤细胞聚集体并产生中风样症状和认知障碍。尽管这两种类型的淋巴瘤中的肿瘤细胞大部分都是 B 细胞来源，但它们的生物学行为不同，并且这种差异背后的详细机制尚不清楚。我们使用免疫组织化学分析研究了原发性中枢神经系统淋巴瘤 (n = 8) 和血管内淋巴瘤 (n = 2) 患者肿瘤组织样本中粘附分子细胞间粘附分子-1 (ICAM-1) 和整合素-β 1 的表达水平。我们还评估了基质金属蛋白酶 (MMP)-2 和 MMP-9 的表达。在 8 名 PCNSML 患者中，6 名患者的 ICAM-1 呈阳性，7 名患者的整合素-β 1 呈阳性。 MMP-2 和 MMP-9 在所有 PCNSML 中均表达。相比之下，两个 IVL 病例中均未呈阳性。我们的研究结果表明，粘附分子和 MMP 对于恶性淋巴瘤细胞从脉管系统侵入脑实质至关重要，并且它们可能是恶性淋巴瘤细胞表现出 PCNSML 或 IVL 细胞的关键决定因素。
Two distinct forms of malignant lymphomas can invade the central nervous system (CNS). Although primary CNS malignant lymphomas (PCNSMLs) invade the brain parenchyma, intravascular lymphomas (IVLs) form tumor cell aggregates in the vasculature and produce stroke-like symptoms and cognitive impairment. Although the tumor cells are mostly of B-cell origin in both types of lymphoma, their biological behavior is different, and the detailed mechanism(s) underlying this difference are not well understood. We studied the expression level of the adhesion molecules intercellular adhesion molecule-1 (ICAM-1) and integrin-beta 1 in tumor tissue samples from patients with primary CNS lymphoma (n = 8) and intravascular lymphoma (n = 2) using immunohistochemical analysis. We also assessed the expression of the matrix metalloproteinases (MMP)-2 and MMP-9. ICAM-1 was positive in six and integrin-beta 1 was positive in seven patients among eight PCNSML patients. MMP-2 and MMP-9 were expressed in all PCNSML. In contrast, none of them was positive in both IVL cases. Our findings suggest that adhesion molecules and MMPs are essential for malignant lymphoma cell invasion from the vasculature into the brain parenchyma and that they may be the key determinants for malignant lymphoma cells to behave as PCNSML or IVL cells.