DETECTION OF MAMMALIAN CARCINOGENS WITH AN IMMUNOLOGICAL DNA SYNTHESIS-INHIBITION TEST
DETECTION OF MAMMALIAN CARCINOGENS WITH AN IMMUNOLOGICAL DNA SYNTHESIS-INHIBITION TEST
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DOI：
10.1093/carcin/13.12.2389
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发表时间：
1992-12-01
期刊：
影响因子：
4.7
通讯作者：
REIFFERSCHEID, G
中科院分区：
文献类型：
--
作者：
HEIL, J;REIFFERSCHEID, G
遗传毒性、致突变性和致癌性之间有着密切的关系。但关于哪种短期检测系统最能识别人类致癌物质的争议仍在继续。目前，沙门氏菌基因突变试验(‘Ames试验’)是目前应用最广泛的诱变剂筛选试验。然而，许多体外测试的有效性数据并不令人满意，这可能是因为目前的短期测试无法检测到非遗传毒性致癌物，这些致癌物正日益成为癌症发生机制讨论的焦点。在这方面经常被忽视的一个原则是，基因毒剂的性质是抑制(增殖的)真核细胞中复制的DNA合成。我们认为这种对DNA损伤的早期反应在癌变的多阶段过程中是重要的。因此，我们建议在测试电池中包括DNA合成抑制测试，以进行致癌物筛选。在本文中，我们报告了一种基于免疫学技术的合适的DNA合成抑制试验的发展。
There is a close relationship between genotoxicity mutagenicity and carcinogenicity. But the controversy of which short-term test system best recognizes human carcinogens is still going on. Currently, the Salmonella gene mutation assay ('Ames test') is the most widely used test for the screening of mutagens. However, many in vitro tests hold unsatisfactory validity data, presumably because of the inability of present short-term tests to detect non-genotoxic carcinogens, which are increasingly being brought into focus in the discussions of genesis of cancer. One principle often neglected in this context is the property of genotoxic agents to inhibit replicative DNA synthesis in (proliferating) eukaryotic cells. We believe that this early response to DNA damage is important in the multistage process of carcinogenesis. Accordingly, we proposed that a DNA synthesis-inhibition test should be included in the test batteries for carcinogen screening. In this paper we report the development of an appropriate DNA synthesis-inhibition test based on immunological techniques.