MutaRNA: analysis and visualization of mutation-induced changes in RNA structure
MutaRNA: analysis and visualization of mutation-induced changes in RNA structure
复制标题
MutaRNA：突变引起的 RNA 结构变化的分析和可视化
DOI：
10.1093/nar/gkaa331
复制
发表时间：
2020
影响因子：
14.9
通讯作者：
Rolf Backofen
中科院分区：
文献类型：
--
作者：
Milad Miladi;Martin Raden;Sven Diederichs;Rolf Backofen
由于其核苷酸之间的分子内相互作用，RNA分子折叠成复杂的结构。许多非编码RNA和信使RNA的一些顺式调节元件的功能高度依赖于它们的折叠。单核苷酸变体(SNV)和其他类型的突变可以通过改变RNA元件的碱基配对模式来扰乱其天然功能。因此，确定突变对RNA结构的影响是评估突变对细胞内RNA转录后调控和功能影响的关键一步。尽管单核苷酸变异可能会对结构形成产生显著影响，但解释和比较这种影响通常需要专业知识和细致的努力。在这里，我们介绍了MutaRNA，一个以直观和集成的方式可视化和解释突变诱导的RNA结构变化的网络服务器。为此，计算并比较了野生型和突变RNA序列的碱基配对概率和位置不配对概率。与圆形图和弧形图相结合的差值热图样点图表示法有助于识别标准输出中隐藏的局部结构混淆。最后，MutaRNA对突变引起的碱基配对潜力和可及性的变化提供了一个全面和比较的概述。MutaRNAWeb服务器可在http://rna.informatik.uni-freiburg.de/MutaRNA.上免费获得
RNA molecules fold into complex structures as a result of intramolecular interactions between their nucleotides. The function of many non-coding RNAs and some cis-regulatory elements of messenger RNAs highly depends on their fold. Single-nucleotide variants (SNVs) and other types of mutations can disrupt the native function of an RNA element by altering its base pairing pattern. Identifying the effect of a mutation on an RNA’s structure is, therefore, a crucial step in evaluating the impact of mutations on the post-transcriptional regulation and function of RNAs within the cell. Even though a single nucleotide variation can have striking impacts on the structure formation, interpreting and comparing the impact usually needs expertise and meticulous efforts. Here, we present MutaRNA, a web server for visualization and interpretation of mutation-induced changes on the RNA structure in an intuitive and integrative fashion. To this end, probabilities of base pairing and position-wise unpaired probabilities of wildtype and mutated RNA sequences are computed and compared. Differential heatmap-like dot plot representations in combination with circular plots and arc diagrams help to identify local structure abberations, which are otherwise hidden in standard outputs. Eventually, MutaRNA provides a comprehensive and comparative overview of the mutation-induced changes in base pairing potentials and accessibility. The MutaRNA web server is freely available at http://rna.informatik.uni-freiburg.de/MutaRNA.
登录
查看更多内容
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
14.9
作者：
Chursov A;Frishman D;Shneider A
通讯作者：
Shneider A
Messenger RNA结构调节翻译起始：从细菌到人类的一种机制。
影响因子：
2.9
作者：
Mustoe AM;Corley M;Laederach A;Weeks KM
通讯作者：
Weeks KM
影响因子：
14.9
作者：
Salari R;Kimchi-Sarfaty C;Gottesman MM;Przytycka TM
通讯作者：
Przytycka TM
显性高铁蛋白血症和白内障家族中 L 铁蛋白 mRNA 铁反应元件的突变
影响因子：
30.8
作者：
C. Beaumont;P. Leneuve;I. Devaux;J. Scoazec;M. Berthier;Marie;B. Grandchamp;D. Bonneau
通讯作者：
D. Bonneau