One-bead-one-structure combinatorial libraries.
One-bead-one-structure combinatorial libraries.
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一珠一结构组合文库。
DOI：
10.1002/bip.360370303
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Lam,KS
中科院分区：
文献类型：
--
作者：
Lebl,M;Krchnák,V;Sepetov,NF;Seligmann,B;Strop,P;Felder,S;Lam,KS
采用一个珠子一个化合物技术的组合库进行审查。这种技术有两个显著特点。首先，每种化合物都用独特的固体支持物鉴定，使活性化合物易于分离。第二，只有在确定其生物学相关性后，才能阐明阳性反应珠上化合物的身份。直接的结构鉴定方法（埃德曼降解和质谱）以及间接的“编码”方法，促进非肽库的合成和筛选进行了讨论。Nonpeptide和“支架”库，连同一个新的方法发现的pentide结合基序使用“库”，也进行了讨论。此外，使用组合库来优化最初发现的线索的能力用使用肽库的实例来说明。John Wiley & Sons，Inc.
Combinatorial libraries employing the one‐bead–one‐compound technique are reviewed. Two distinguishing features characterize this technique. First, each compound is identified with a unique solid support, enabling facile segregation of active compounds. Second, the identity of a compound on a positively reacting bead is elucidated only after its biological relevance is established. Direct methods of structure identification (Edman degradation and mass spectroscopy) as well as indirect “coding” methods facilitating the synthesis and screening of nonpeptide libraries are discussed. Nonpeptide and “scaffold” libraries, together with a new approach for the discovery of a pentide binding motif using a “library of libraries,” are also discussed. In addition, the ability to use combinatorial libraries to optimize initially discovered leads is illustrated with examples using peptide libraries. © 1994 John Wiley & Sons, Inc.