Enzyme electrodes stabilized by monolayer-modified nanoporous Au for biofuel cells
Enzyme electrodes stabilized by monolayer-modified nanoporous Au for biofuel cells
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DOI：
10.1007/s13404-011-0038-1
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发表时间：
2012-01-01
期刊：
影响因子：
2.2
通讯作者：
Mabuchi, Mamoru
中科院分区：
文献类型：
--
作者：
Hakamada, Masataka;Takahashi, Masaki;Mabuchi, Mamoru
采用Au- ag脱合金法制备孔径约为40 nm的开孔纳米孔Au （np-Au）电极，并在电极表面修饰4-氨基噻吩自组装单层（SAM），以提高固定化漆酶和葡萄糖氧化酶的电催化活性。酶固定的sam修饰的np-Au工作电极在缓冲溶液（pH = 5.0）的循环伏安图中显示出额外的还原氧化峰对。因此，在np-Au上的SAM促进了电极和反应物之间的电子转移。完美和缺陷Au（111）表面的第一性原理计算表明，np-Au纳米结构表面的原子缺陷是电子转移增强的关键原因。为了利用这些结果，初步制备了由酶固定的SAM修饰的np-Au电极组成的葡萄糖/O-2生物燃料电池，在20℃下其最大功率密度为52 μ W/cm(2)。进一步优化纳米孔结构和SAM种类将提高生物燃料电池的性能。
Open-cell nanoporous Au (np-Au) electrodes with pore size of approximately 40 nm were fabricated by dealloying of Au-Ag, and surfaces of the electrodes were modified with a self-assembled monolayer (SAM) of 4-aminothiophenol to enhance the electrocatalytic activities of immobilized laccase and glucose oxidase. Enzymeimmobilized SAM-modified np-Au working electrodes exhibited additional reduction-oxidation peak pairs in cyclic voltammograms in buffer solution (pH = 5.0). Thus, the SAM on the np-Au facilitated electron transfer between the electrode and reactants. First-principles calculations of perfect and defective Au (111) surfaces indicated that the atomic defects at nanoligament surface of np-Au are critically responsible for the electron transfer enhancement. For the utilization of these results, a glucose/O-2 biofuel cell composed of these enzyme-immobilized SAM-modified np-Au electrodes was preliminarily fabricated, and it exhibited a maximum power density of 52 mu W/cm(2) at 20 degrees C. Further optimization of nanoporous structures and kinds of SAM will improve the performance of biofuel cells.