Intracellular ice formation is affected by cell interactions
Intracellular ice formation is affected by cell interactions
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DOI：
10.1006/cryo.1999.2179
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发表时间：
1999-06-01
期刊：
影响因子：
2.7
通讯作者：
McGann, LE
中科院分区：
文献类型：
--
作者：
Acker, JP;Larese, A;McGann, LE
细胞与细胞和细胞与表面的相互作用对组织的结构和功能很重要。这些相互作用也是组织低温反应的重要决定因素。四个在体外模型中使用仓鼠成纤维细胞在组织培养中被用来研究细胞-细胞和细胞-表面相互作用对这些系统中细胞内冰形成的影响。这四种模式是：（a）悬浮液中的单细胞;（B）仅通过细胞-表面粘附而单独附着于玻璃的细胞;（c）通过细胞-细胞和细胞-表面相互作用而附着于玻璃的细胞集落;和（d）具有广泛的细胞-细胞接触的多细胞球状体。冷冻显微镜用于监测冰成核后细胞外溶液中的细胞内冰形成的流行率和动力学。50%的细胞的细胞内冷冻温度受到细胞-细胞和细胞-表面相互作用的显著影响。也有证据表明，细胞间的核通过细胞间的相互作用。结果表明，细胞-细胞和细胞-表面相互作用在组织系统的低温响应中起着重要作用。(C)北京：科学出版社.
Cell-to-cell and cell-to-surface interactions are important to the structure and function of tissues. These interactions are also important determinants of low-temperature responses in tissues. Four in vitro models using hamster fibroblast cells in tissue culture were used to investigate the influence of cell-cell and cell-surface interactions on intracellular ice formation in these systems. The four models were: (a) single cells in suspension; (b) cells individually attached to glass with only cell-to-surface adhesion; (c) colonies of cells attached to glass with both cell-cell and cell-surface interactions; and (d) multicellular spheroids with extensive cell-cell contacts. Cryomicroscopy was used to monitor the prevalence and kinetics of intracellular ice formation after ice nucleation in the extracellular solution. The temperature for intracellular freezing in 50% of the cells was significantly affected by both cell-cell and cell-surface interactions. There was also evidence of intercellular nucleation through cell-cell interactions. The results indicate that cell-cell and cell-surface interactions play a significant role in the low-temperature response of tissue systems. (C) 1999 Academic Press.