Trace elements in human seminal plasma and spermatozoa.
Trace elements in human seminal plasma and spermatozoa.
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人类精浆和精子中的微量元素。
DOI：
10.1016/0009-8981(83)90019-0
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
D. Mei
中科院分区：
文献类型：
--
作者：
P. Pleban;D. Mei
人精浆和精子中镉、铜、铁、铅、硒和锌的分析方法已经发展。直接在精浆稀释液或冻干细胞硝酸消化液中使用塞曼效应原子吸收光谱法进行分析。合并标本的运行内变异系数（CV）范围为0.5%-9%。运行间CV范围为4%-13%。在1 2，1，1 1 2，3 1 2，和5小时后排放的精浆标本的分析表明，精浆微量元素浓度没有发生变化，站在与精子接触。微量元素的浓度进行了测定，从患者进行不孕症研究的标本。
Methodologies for the analysis of cadmium, copper, iron, lead, selenium and zinc in human seminal plasma and spermatozoa have been developed. Analyses were made directly in a dilution of seminal plasma or nitric acid digest of lyophilized cells using Zeeman-effect atomic absorption spectroscopy. Within-run coefficients of variation (CV's) for pooled specimens ranged from 0.5% to 9%. Between-run CV's ranged from 4% to 13%. Analysis of a seminal plasma specimen at 1 2, 1, 1 1 2, 3 1 2, and 5 hours post emission indicated that no change in seminal plasma trace element concentrations occurred on standing in contact with spermatozoa. Trace element concentrations were determined in specimens from patients undergoing infertility studies.