Fibroblast growth factors 1 and 2 in the primate uterus
Fibroblast growth factors 1 and 2 in the primate uterus
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DOI：
10.1095/biolreprod59.3.491
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发表时间：
1998-09-01
影响因子：
3.6
通讯作者：
Bondy, CA
中科院分区：
文献类型：
--
作者：
Samathanam, CA;Adesanya, OO;Bondy, CA
成纤维细胞生长因子（FGF）1和2是许多组织中增殖和血管生成的旁分泌效应物。为了阐明这些生长因子在子宫可塑性中的潜在作用，我们使用原位杂交组织化学来鉴定FGF-1和FGF-2产生的细胞来源，并使用免疫组织化学来鉴定灵长类动物子宫中肽的细胞和细胞外沉积位点。为了评价雌二醇对子宫FGF的影响，研究了用含雌二醇或载体的颗粒处理的卵巢切除恒河猴的子宫。FGF-1和FGF-2 mRNA均表达于子宫上皮细胞和子宫肌层细胞。使用计算机颗粒计数的mRNA水平的定量比较显示，雌二醇和车辆治疗的动物之间没有显着差异。FGF-1免疫反应检测到分散的上皮细胞，血管，子宫肌层细胞在车辆治疗的动物，但发现在雌二醇治疗的动物显着更强烈和广泛。在这两种情况下，FGF-1免疫染色主要是核。FGF-2的免疫反应集中在细胞外的基底层的腺上皮和表面，是丰富的和弥漫性分布在子宫肌层和血管细胞的细胞质和细胞核。与雌二醇治疗相关的FGF-2免疫染色的模式或强度没有明显差异。这些数据表明，主要的子宫细胞类型合成FGF-1和-2，这两种肽是差异定位在子宫细胞和细胞外室和差异敏感的调节雌二醇。
Fibroblast growth factors (FGF) 1 and 2 are paracrine effecters of proliferation and angiogenesis in many tissues. To elucidate potential roles for these growth factors in uterine plasticity, we used in situ hybridization histochemistry to identify the cellular sources of FGF-1 and -2 production, and immunohistochemistry to identify the cellular and extracellular deposition sites of the peptides in the primate uterus. To evaluate the effects of estradiol on uterine FGFs, uteri from ovariectomized rhesus monkeys treated with estradiol- or vehicle-containing pellets were investigated. FGF-1 and -2 mRNAs were both expressed in uterine epithelial and myometrial cells. Quantitative comparison of their mRNA levels using computerized grain counting showed no significant difference between estradiol- and vehicle-treated animals.FGF-1 immunoreactivity was detected in scattered epithelial, vascular, and myometrial cells in the vehicle-treated animals but found to be significantly more intense and widespread in estradiol-treated animals. In both conditions, FGF-1 immunostaining was predominantly nuclear. FGF-2 immunoreactivity was concentrated extracellularly in the basal lamina of both glandular and surface epithelium and was abundant and diffusely distributed within myometrial and vascular cells in both cytoplasm and nucleus. There was no apparent difference in the pattern or intensity of FGF-2 immunostaining related to estradiol treatment. These data demonstrate that major uterine cell types synthesize both FGF-1 and -2, and that the two peptides are differentially localized in uterine cellular and extracellular compartments and differentially sensitive to regulation by estradiol.