The hypersensitive induced reaction 3 (HIR3) gene contributes to plant basal resistance via an EDS1 and salicylic acid-dependent pathway
The hypersensitive induced reaction 3 (HIR3) gene contributes to plant basal resistance via an EDS1 and salicylic acid-dependent pathway
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过敏诱导反应 3 (HIR3) 基因通过 EDS1 和水杨酸依赖性途径促进植物基础抗性
DOI：
10.1111/tpj.14271
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发表时间：
2019-06-01
期刊：
影响因子：
7.2
通讯作者：
Yan, Fei
中科院分区：
文献类型：
--
作者：
Li, Saisai;Zhao, Jinping;Yan, Fei
超敏诱导反应（hypersensitive-induced reaction，HIR）基因家族与植物超敏反应（hypersensitiveresponse，HR）有关，HR是植物抵抗细菌和真菌病原体防御系统的一部分。HIR基因参与对病毒病原体的应答尚未研究。我们现在报告，本氏烟草和水稻（水稻）的HIR 3基因上调水稻条纹病毒（RSV）感染后。沉默N.本塞姆氏烟（N. benthamiana）导致RSV RNA积累增加，而HIR 3s在本塞姆氏烟（N.本塞姆氏菌或水稻降低了RSV RNA的表达并降低了症状的严重程度，同时还赋予了对芜菁花叶病毒、马铃薯X病毒以及细菌病原体假单胞菌和黄单胞菌的抗性。在N.本塞姆那降低了水杨酸（SA）的含量，并伴随着SA途径中基因表达的下调。瞬时表达的两个HIR 3基因同源物从N. benthamiana或水稻HIR 3基因。benthamiana叶引起细胞死亡和SA的积累，但在EDS 1沉默的植物或表达NahG的植物中没有这样做。结果表明，HIR 3通过EDS 1和SA依赖的途径参与植物的基础抗性。
The hypersensitive-induced reaction (HIR) gene family is associated with the hypersensitive response (HR) that is a part of the plant defense system against bacterial and fungal pathogens. The involvement of HIR genes in response to viral pathogens has not yet been studied. We now report that the HIR3 genes of Nicotiana benthamiana and Oryza sativa (rice) were upregulated following rice stripe virus (RSV) infection. Silencing of HIR3s in N. benthamiana resulted in an increased accumulation of RSV RNAs, whereas overexpression of HIR3s in N. benthamiana or rice reduced the expression of RSV RNAs and decreased symptom severity, while also conferring resistance to Turnip mosaic virus, Potato virus X, and the bacterial pathogens Pseudomonas syringae and Xanthomonas oryzae. Silencing of HIR3 genes in N. benthamiana reduced the content of salicylic acid (SA) and was accompanied by the downregulated expression of genes in the SA pathway. Transient expression of the two HIR3 gene homologs from N. benthamiana or the rice HIR3 gene in N. benthamiana leaves caused cell death and an accumulation of SA, but did not do so in EDS1-silenced plants or in plants expressing NahG. The results indicate that HIR3 contributes to plant basal resistance via an EDS1- and SA-dependent pathway.