Cell secretion and membrane fusion
Cell secretion and membrane fusion
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DOI：
10.1016/j.domaniend.2005.02.039
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发表时间：
2005-07-01
影响因子：
2.1
通讯作者：
Jena, BP
中科院分区：
文献类型：
--
作者：
Jena, BP
分泌发生在多细胞生物体的所有细胞中，并且涉及将包装在膜结合囊泡中的分泌产物递送到细胞外部。专门用于神经传递、酶分泌或激素释放的细胞利用高度调节的分泌过程。分泌囊泡被运输到质膜上的特定位点，在那里它们停靠并融合以释放它们的内容物。与其他细胞过程类似，细胞分泌被发现是高度调节和精确协调的事件。已经证明，膜结合的分泌囊泡在细胞质膜上的特化超分子结构孔体处对接和融合。分泌囊泡的膨胀导致压力的积聚，从而允许囊泡内内容物的排出。分泌囊泡肿胀的程度决定了分泌过程中排出的囊内内容物的量。多孔体的发现，它的分离，它的结构和动力学在nm分辨率和真实的时间，它的生化组成和功能重建成人工脂质膜，已被确定。分泌囊泡在孔体基部融合和囊泡肿胀的分子机制也得到了解决。这些发现揭示了细胞分泌的分子机制。(c)2005年爱思唯尔公司All rights reserved.
Secretion occurs in all cells of multicellular organisms and involves the delivery of secretory products packaged in membrane-bound vesicles to the cell exterior. Specialized cells for neurotransmission, enzyme secretion or hormone release utilize a highly regulated secretory process. Secretory vesicles are transported to specific sites at the plasma membrane, where they dock and fuse to release their contents. Similar to other cellular processes, cell secretion is found to be highly regulated and a precisely orchestrated event. It has been demonstrated that membrane-bound secretory vesicles dock and fuse at porosomes, which are specialized supramolecular structures at the cell plasma membrane. Swelling of secretory vesicles results in a build-up of pressure, allowing expulsion of intravesicular contents. The extent of secretory vesicle swelling dictates the amount of intravesicular contents expelled during secretion. The discovery of the porosome, its isolation, its structure and dynamics at nm resolution and in real time, its biochemical composition and functional reconstitution into artificial lipid membrane, have been determined. The molecular mechanism of secretory vesicle fusion at the base of porosomes, and vesicle swelling, has also been resolved. These findings reveal the molecular mechanism of cell secretion. (c) 2005 Elsevier Inc. All rights reserved.