A simple rapid radiometric assay for pyrimidine-5'-nucleotidase.
A simple rapid radiometric assay for pyrimidine-5'-nucleotidase.
复制标题
嘧啶-5-核苷酸酶的简单快速放射测定。
DOI：
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发表时间：
1977
期刊：
影响因子：
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通讯作者：
E. Beutler
中科院分区：
文献类型：
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作者：
J. Torrance;C. West;E. Beutler
嘧啶-5'-核苷酸酶缺乏似乎是与红细胞嗜碱性点滴相关的溶血性贫血的重要原因。已经开发出一种用于测定这种酶的新辐射测定方法。在该技术中，14C-CMP用作衬底。未脱磷酸化为胞苷的 CMP 与脱蛋白过程中形成的硫酸钡沉淀结合。胞苷保留在溶液中并进行计数。该方法简单、重现性好，可对大量样品进行测定。通过该技术已检测到两名患有嘧啶-5'-核苷酸酶缺乏症的患者。
Pyrimidine-5'-nucleotidase deficiency appears to be an important cause of hemolytic anemia associated with basophilic stippling of the red cells. A new radiometric method for the assay of this enzyme has been developed. In this technique, 14C-CMP serves as substrate. The CMP which is not dephosphorylated to cytidine is bound to the barium sulfate precipitate which forms in the deproteinization process. The cytidine remains in solution and is counted. The method is simple and reproducible and can be carried out on large numbers of samples. Two patients with pyrimidine-5'-nucleotidase deficiency have been detected by means of this technique.