DNA mediated sequential self-assembly of nano/micro components
DNA mediated sequential self-assembly of nano/micro components
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DNA介导的纳米/微米组件的顺序自组装
DOI：
10.1109/memsys.2008.4443840
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Osamu Tabata
中科院分区：
文献类型：
--
作者：
T. Kusakabe;T. Tanemura;Y. Higuchi;K. Sugano;T. Tsuchiya;Osamu Tabata
首次提出了一种应用脱氧核糖核酸（DNA）杂交技术进行纳米/微组分序列自组装的新方法，并对其有效性进行了实验验证。在该过程中，DNA的特性，如互补对单链DNA （ssDNA）杂交成双链DNA （dsDNA）的特异性，以及杂交对环境温度（熔融温度Tm）的依赖性，发挥了关键作用。DNA的这些特征可以通过DNA碱基对序列来设计和合成。采用两种不同Tm（65℃和50℃）的ssDNA进行了三种自组装实验；1.)金纳米粒子之间，2.)金纳米粒子与镀金衬底之间，3.)硅微组件（5times3mum3）与镀金衬底之间。通过这些实验，成功地验证了可以通过控制环境温度来控制自组装的顺序。这一过程的可逆性也得到了证实。然而，硅微元件在镀金衬底上的组装尚未得到证实。
A novel sequential self-assembly process of nano/micro components applying deoxyribonucleic acid (DNA) hybridization was proposed for the first time and its validity was experimentally verified. In the proposed process, characteristics of DNA, such as hybridization specificity of complementary pairs of single-stranded DNA (ssDNA) into a double-stranded DNA (dsDNA), and the dependence of hybridization on ambient temperature (melting temperature Tm) plays a key role. These characteristics of DNA can be designed and synthesized by base-pair sequence of DNA. Three types of self-assembly experiments using two types of ssDNA with different Tm (65degC and 50degC) were carried out; 1.) between Au nanoparticles, 2.) between Au nanoparticle and Au coated substrate, and 3.) between silicon micro components (5times5times3 mum3) and Au coated substrate. Through these experiments, it was successfully verified that the sequence of the self-assembly can be controlled by controlling the ambient temperature. The reversibility of this process was also confirmed. However, the silicon micro components assembly on Au coated substrate was not confirmed.