The fission yeast meiotic regulator Mei2p forms a dot structure in the horse-tail nucleus in association with the sme2 locus on chromosome II
The fission yeast meiotic regulator Mei2p forms a dot structure in the horse-tail nucleus in association with the sme2 locus on chromosome II
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DOI：
10.1091/mbc.e02-11-0738
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发表时间：
2003-06-01
影响因子：
3.3
通讯作者：
Yamamoto, M
中科院分区：
文献类型：
--
作者：
Shimada, T;Yamashita, A;Yamamoto, M
裂解酵母Mei2p是一种RNA结合蛋白，对诱导前减数分裂DNA合成和第一次减数分裂是必不可少的。在减数分裂前期，Mei2p在马尾核的一个明显固定的位置形成点状结构。这种点的形成需要一个减数分裂特异的RNA物种，meiRNA，这是减数分裂I所必需的，而这个点的出现是细胞进行减数分裂I的能力的指示器。利用单倍体减数分裂中的染色体分离、染色体的相互易位和基因易位的分析，我们得出结论：Mei2p点与编码meiRNA的第二染色体短臂上的sme2基因有关。Mei2p点定位的决定因素似乎是sme2的转录本，而不是该基因的DNA序列。然而，证据表明，这个点可能不是Mei2p与meiRNA转录过程中附着的简单反映。我们推测，Mei2p点是一种特化结构，或者是为了促进Mei2p和meiRNA的组装，或者是为了执行一些减数分裂I所必需的未知功能。
Fission yeast Mei2p is an RNA-binding protein essential for induction of both premeiotic DNA synthesis and first meiotic division. Mei2p forms a dot structure at an apparently fixed position in the horse-tail nucleus during meiotic prophase. This dot formation requires a meiosis-specific RNA species, meiRNA, which is indispensable for meiosis I, and the emergence of the dot is an indicator of the ability of the cell to perform meiosis I. Herein, we have sought the identity of this dot. Analyses using chromosome segregation in haploid meiosis, reciprocal translocation of chromosomes, and gene translocation have led us to conclude that the Mei2p dot is in association with the sme2 gene on the short arm of chromosome II, which encodes meiRNA. Transcripts of sme2, rather than the DNA sequence of the gene, seem to be the determinant of the localization of the Mei2p dot. However, evidence suggests that the dot may not be a simple reflection of the attachment of Mei2p to meiRNA undergoing transcription. We speculate that the Mei2p dot is a specialized structure, either to foster the assembly of Mei2p and meiRNA or to perform some unidentified function indispensable for meiosis I.