Scratching the Surface—How Decisive Are Microscopic Surface Structures on Growth and Performance of Electrochemically Active Bacteria?
Scratching the Surface—How Decisive Are Microscopic Surface Structures on Growth and Performance of Electrochemically Active Bacteria?
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DOI：
10.3389/fenrg.2019.00018
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发表时间：
2019-03
影响因子：
3.4
通讯作者：
Christopher Moß;S. Patil;U. Schröder
中科院分区：
文献类型：
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作者：
Christopher Moß;S. Patil;U. Schröder
本研究阐明了微米尺度电极表面结构对混合培养电化学活性生物膜（EAB）生长和电化学性能的影响。为此，利用共聚焦激光扫描显微镜（CLSM）对铜电极进行了加工，产生了从几μm到100μm以上的微尺度表面结构（粗糙度和波纹度）。结构电极用于培养醋酸基、混合培养、阳极EAB，以建立表面性质与(i)生长行为和（ii）所得EAB的固定电催化性能之间的关系。在短时间尺度上，初始生长阶段的行为受到表面拓扑结构的显著影响。然而，电催化生物膜的长期性能不表现出对表面结构的任何依赖，因此不能从三维空间的增加而增加的比表面积和微尺度表面积中获益。本研究的结果对于更系统地开发微生物电化学技术的定制电极具有重要意义。
This study elucidates the role of micrometer-scale electrode surface structures on the growth and the electrochemical performance of mixed culture electrochemically active biofilms (EAB). For this purpose, copper electrodes were machined to generate micro-scale surface structures (roughness and waviness) ranging from a few μm to over 100μm, which were characterized using confocal laser scanning microscopy (CLSM). The structured electrodes were used to cultivate acetate based, mixed culture, anodic EAB in order to establish relationships between the surface properties and (i) the growth behavior and (ii) the stationary electrocatalytic properties of the resulting EAB. On short time scale, the initial growth phase is shown to be significantly influenced behavior by the surface topology. The long term electrocatalytic biofilm performance, however, does not show any dependence on the surface structures and does thus not profit from the increased specific surface area and micro-scale surface area due to the increasing 3-dimensionality. The results of this study are of great importance for a more systematic development of tailored electrodes for microbial electrochemical technologies.