An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits.
An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits.
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A型肉毒毒素治疗家兔颌下腺移植术后分泌过多的实验研究
DOI：
10.1155/2016/7058537
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发表时间：
2016
影响因子：
1.9
通讯作者：
Cai ZG
中科院分区：
文献类型：
--
作者：
Xie S;Xu H;Lin B;Wang K;Shan XF;Cai ZG
目标.目的探讨A型肉毒毒素（BTXA）对兔颌下腺移植后分泌过多的抑制作用及其可能机制。方法.建立了一种新的SMG移植模型。将30只造模成功的大鼠随机分为5组，包括对照组和4个实验组。采用分泌量分析BTXA注射对过度分泌的影响。通过HE染色、透射电镜、免疫印迹和免疫荧光等方法分析其可能的机制。结果注射BTXA后，SMG移植后2周和4周组的过度分泌明显减少，但对12周和24周组无明显影响。HE和TEM结果显示，BTXA可引起移植SMG腺泡细胞形态和超微结构的改变。Western blot结果显示，BTXA注射后2周和4周，水通道蛋白5（AQP5）蛋白表达下降。免疫荧光结果显示，BTXA注射后2周和4周AQP5蛋白主要表达于胞浆，提示BTXA促进AQP5表达由细胞膜向胞浆转移。结论BTXA能有效地控制SMG移植后的过度分泌。
Objectives. To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms. Methods. A new SMG transplantation model was established in rabbit. 30 successfully constructed models were randomly assigned to five groups including control group and four experimental groups. Secretion outputs were used to analyze the effect of BTXA injection on excessive secretion. Hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), Western blot, and immunofluorescence were performed to analyze its possible mechanisms. Results. After BTXA injection, a significant decrease of excessive secretion after SMG transplantation was found in 2 and 4 weeks groups, but no significant effect on 12 and 24 weeks groups. HE and TEM results showed that BTXA led to morphological and ultrastructural changes of acinar cells of transplanted SMG. Western blot results suggested that BTXA decreased the aquaporin-5 (AQP5) protein expression after BTXA injection for 2 and 4 weeks. Immunofluorescence results showed that AQP5 protein was mainly expressed in the cytoplasm after BTXA injection for 2 and 4 weeks, which might indicate that BTXA promoted AQP5 expression from the cell membrane to cytoplasm. Conclusion. BTXA could effectively control excessive secretion after SMG transplantation in rabbits.
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影响因子：
3.1
作者：
Jacobsen, Hans-Christian;Hakim, Samer G.;Sieg, Peter
通讯作者：
Sieg, Peter
影响因子：
4.8
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Ma, TH;Song, YL;Verkman, AS
通讯作者：
Verkman, AS
影响因子：
4.2
作者：
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通讯作者：
Geerling, Gerd
影响因子：
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作者：
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通讯作者：
Nakao, Kumiko
影响因子：
4.4
作者：
Lin, PY;Cheng, CY;Chou, P
通讯作者：
Chou, P