EFFECTS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) ON OVULATION IN THE RAT OVARY
EFFECTS OF TUMOR-NECROSIS-FACTOR-ALPHA (TNF-ALPHA) ON OVULATION IN THE RAT OVARY
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DOI：
10.1071/rd9950067
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发表时间：
1995-01-01
影响因子：
1.9
通讯作者：
NORMAN, RJ
中科院分区：
文献类型：
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作者：
BRANNSTROM, M;BONELLO, N;NORMAN, RJ
在体外灌流20h的大鼠卵巢上，观察肿瘤坏死因子对排卵率、卵巢类固醇分泌及排卵介质纤溶酶原激活物(PA)和前列腺素的影响。肿瘤坏死因子α(40ngmL(-1))诱导排卵3/5卵巢(0.8+/-0.4)。当在促黄体生成素的同时添加肿瘤坏死因子α时，促黄体生成素的排卵率提高了4倍(10.6+/-1.3)。而在LH5h后加入肿瘤坏死因子α(3.2+/-0.6)则无此作用。与单用促黄体生成素相比，肿瘤坏死因子α与促黄体生成素联合应用时孕酮的释放更多。组织中前列腺素F-2α(PGF(2α))和前列腺素E(PGE)的浓度在加入肿瘤坏死因子α后较对照组升高，但未超过联合应用时的水平。组织中PA活性不受肿瘤坏死因子α的影响，当肿瘤坏死因子α与黄体生成素联合应用时，可抑制黄体生成素诱导的前列腺素A活性升高。结果表明，促炎性细胞因子肿瘤坏死因子α促进大鼠卵巢排卵。
The effects of tumour necrosis factor alpha (TNF alpha) on ovulation rate, ovarian secretion of steroids, and on tissue concentrations of the ovulatory mediators plasminogen activator (PA) and prostaglandins were examined in rat ovaries perfused in vitro for 20 h. Unstimulated control ovaries did not ovulate whereas luteinizing hormone (LH; 100 ng mL(-1)) induced ovulations in all ovaries (2.6+/-0.7). TNF alpha (40 ng mL(-1)) induced ovulations in 3 of 5 ovaries (0.8+/-0.4). When TNF alpha was added concomitantly with LH, the LH-induced ovulation rate was increased four-fold (10.6+/-1.3). This effect was not seen when TNF alpha was added 5 h after LH (3.2+/-0.6). More progesterone was released when TNF alpha was combined with LH by comparison with LH alone. Tissue concentrations of prostaglandin F-2 alpha (PGF(2 alpha)) and PGE were increased by the addition of TNF alpha when compared with the control group, but did not exceed those observed in the LH group, when TNF alpha was combined with LH. PA activity in the tissue was unaffected by TNF alpha and the LH-induced increase in PA activity was inhibited when TNF alpha was combined with LH. The results demonstrate that the proinflammatory cytokine TNF alpha promotes ovulations in the rat ovary.