Comparison of micafungin and fluconazole for experimental Candida keratitis in rabbits
Comparison of micafungin and fluconazole for experimental Candida keratitis in rabbits
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DOI：
10.1097/ico.0b013e31802cd8a8
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发表时间：
2007-04-01
期刊：
影响因子：
2.8
通讯作者：
Oshika, Tetsuro
中科院分区：
文献类型：
--
作者：
Hiraoka, Takahiro;Kaji, Yuichi;Oshika, Tetsuro
目的：目的：观察米卡芬净结膜下注射治疗兔实验性白念珠菌性角膜炎的疗效，并与氟康唑进行比较。将白色念珠菌（5 × 10（4）酵母细胞）接种于角膜基质中。将动物随机分配至3组，并接受0.5 mL 0.1%米卡芬净、0.2%氟康唑或生理盐水结膜下注射，每天一次，持续3周。裂隙灯生物显微镜和组织病理学检查的眼睛。比较3组真菌性角膜炎的临床病程。结果：米卡芬净组的临床评分明显低于其他两组（P < 0.0001，P = 0.0027，Bonferroni多重比较）。氟康唑组在第18天的临床评分显著低于生理盐水组（P = 0.0343）。在研究期结束时，生理盐水和米卡芬净组（P < 0.0001）、生理盐水和氟康唑组（P = 0.0072）、氟康唑和米卡芬净组（P = 0.0013）之间存在显著差异。组织学上，获得了类似的结果。此外，微生物检查的结果几乎匹配的临床和组织病理学findings.Conclusions：结膜下给药米卡芬净是有效的治疗实验性念珠菌性角膜炎。米卡芬净局部应用于眼部将是临床真菌性角膜炎的一种可行的治疗选择。
Purpose: To evaluate the efficacy of subconjunctival injection of micafungin in the treatment of experimental Candida albicans keratitis in rabbits compared with fluconazole.Methods: In 1 eye of 24 New Zealand white rabbits, C. albicans (5 X 10(4) yeast cells) was inoculated in the corneal stroma. The animals were randomly assigned to 3 groups and received subconjunctival injection of 0.5 mL of 0.1% micafungin, 0.2% fluconazole, or physiologic saline once a day for 3 weeks. The eyes were examined slit-lamp biomicroscopically and histopathologically. The clinical course of fungal keratitis was compared among the 3 groups. In another 36 rabbits, a microbiological examination was performed using a quantitative isolate recovery technique, and the numbers of colony-forming units were compared among groups.Results: The clinical scores were significantly lower in the micafungin group than in the other 2 groups throughout the study period (P < 0.0001 similar to P = 0.0027, Bonferroni multiple comparison). The fluconazole group showed significantly lower clinical scores than the saline group on day 18 (P = 0.0343). At the end of the study period, there were significant differences between the saline and micafungin groups (P < 0.0001), the saline and fluconazole groups (P = 0.0072), and the fluconazole and micafungin groups (P = 0.0013). Histopathologically, similar results were obtained. Moreover, the results of the microbiological examination nearly matched the clinical and histopathologic findings.Conclusions: Subconjunctival administration of micafungin was effective in the treatment of experimental Candida keratitis. Local application of micafungin to the eye would be a feasible treatment option for clinical fungal keratitis.