Transcriptional regulation of human placental corticotropin-releasing factor by prostaglandins and estradiol.
Transcriptional regulation of human placental corticotropin-releasing factor by prostaglandins and estradiol.
复制标题
前列腺素和雌二醇对人胎盘促肾上腺皮质激素释放因子的转录调节。
DOI：
10.1095/biolreprod57.6.1285
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发表时间：
1997
影响因子：
3.6
通讯作者：
Adler,S
中科院分区：
文献类型：
--
作者：
Dibbs,KI;Anteby,E;Mallon,MA;Sadovsky,Y;Adler,S
The mechanism of labor initiation in humans has not been completely elucidated. Prostaglandins, estrogens, and corticotropin-releasing factor (CRF) have all been shown to affect uterine myocytes and enhance uterine contractility. There are also indications that these uterine regulators have additional effects on other sites involved in labor and that they may act in concert or, perhaps, by regulating each other. Therefore, we evaluated the CRF promoter for transcriptional regulation by prostaglandins and estrogens.Human placental choriocarcinoma cell lines were transfected with CRF-luciferase reporter genes and treated with prostaglandins. Prostaglandin E2(PGE2), but not prostaglandin F2α(PGF2α), stimulated CRF-luciferase expression in choriocarcinoma cell lines via a cAMP-dependent pathway. A combination of transfections and in vitro binding studies tested for potential regulation of CRF by estrogen receptor (ER). ER neither regulated the CRF promoter nor interacted with steroid response half-sites from the CRF promoter.Our results provide a molecular regulatory link between PGE2and CRF, two compounds that enhance uterine contractile function. Combined with the stimulation of prostaglandin release by CRF, these data support a potentially important ″feed-forward″ regulatory loop involving CRF and PGE2 in parturition. In contrast, we found no evidence for direct effects of estrogens or PGF2αon CRF transcription.
