The rauscher‐MuLV‐induced leukemia, RBL‐5, bears two tumor‐associated transplantation antigens expressed on distinct molecules
The rauscher‐MuLV‐induced leukemia, RBL‐5, bears two tumor‐associated transplantation antigens expressed on distinct molecules
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Rauscher-MuLV 诱导的白血病 RBL-5 具有两种在不同分子上表达的肿瘤相关移植抗原
DOI：
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发表时间：
1985
影响因子：
6.4
通讯作者：
M. Rogers
中科院分区：
文献类型：
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作者：
G. Galetto;L. Law;M. Rogers
肿瘤细胞经常在其表面上表达新的抗原决定簇，这使得它们在宿主动物中具有免疫原性。当对这种抗原的免疫导致同源肿瘤移植物的排斥反应时，它被称为肿瘤相关移植抗原（TATA）。RBL-5是一种Rauscher鼠白血病病毒（MuLV）诱导的C57 BI/6来源的白血病，具有强效免疫原性，与密切相关的Friend和Moloney-MuLV（FMR-TATA）诱导的其他肿瘤具有相同的TATA。我们最近已经分离了175千道尔顿（kd）糖蛋白（gp 175），其具有FMR-TATA预期的所有性质（Rogers等人，1984年）。当该分子通过DEAE层析从纯化的总糖蛋白级分中分离时，剩余的糖蛋白仍然含有高度免疫原性的TATA。涉及放射免疫测定和兔抗gp 175免疫沉淀的对照实验表明，这种免疫原性不是由于残留的gp 175或gp 175的分解产物。因此，我们得出结论，RBL-5表达至少两种不同的TATA：gp 175和另一种糖蛋白，通过从二乙基氨基乙基纤维素（DE 52）柱洗脱，与gp 175区分开来。来自完全体内系统的这些结果支持通过体外方法获得的其他肿瘤的数据，并表明肿瘤细胞可能表达几种免疫原性分子。
Tumor cells frequently express on their surface a new antigenic determinant which renders them immunogenic in the host animal. When immunity to this antigen results in rejection of a syngeneic tumor transplant, it is referred to as a tumor‐associated transplantation antigen (TATA). RBL‐5 is a Rauscher murine leukemia virus (MuLV}‐induced leukemia of C57BI/6 origin that is potently immunogenic and shares a TATA with other tumors induced by the closely related Friend and Moloney‐MuLVs (FMR‐TATA). We have recently isolated a 175 kilodalton (kd) glycoprotein (gp175) which has all the properties expected of the FMR‐TATA (Rogers et al., 1984). When this molecule was separated from a purified total glycoprotein fraction by DEAE chromatography, the remaining glycoproteins still contained a highly immunogenic TATA. Control experiments involving radioimmunoassay and immunoprecipitation with rabbit anti‐gp 175 indicated that this immunogenicity was not due to residual gp 175 or breakdown products of gp 175. We therefore conclude that RBL‐5 expresses at least two distinct TATAs: gp175 and another glycoprotein distinguished from gp 175 by its elution from a diethylaminoethylcellulose (DE52) column. These results, from a completely in vivo system, support data with other tumors obtained by in vitro methods and indicate that tumor cells may express several immunogenic molecules.
恶性肿瘤上独立的免疫显性和免疫隐性肿瘤特异性抗原：用溶细胞 T 细胞克隆进行抗原解剖。
DOI：
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发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Wortzel,RD;Urban,JL;Philipps,C;Fitch,FW;Schreiber,H
通讯作者：
Schreiber,H