GLUCOCORTICOSTEROIDS INCREASE LEUCINE OXIDATION AND IMPAIR LEUCINE BALANCE IN HUMANS
GLUCOCORTICOSTEROIDS INCREASE LEUCINE OXIDATION AND IMPAIR LEUCINE BALANCE IN HUMANS
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DOI：
10.1152/ajpendo.1989.257.5.e712
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发表时间：
1989-11-01
影响因子：
--
通讯作者：
HAYMOND, MW
中科院分区：
文献类型：
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作者：
BEAUFRERE, B;HORBER, FF;HAYMOND, MW
高剂量糖皮质激素治疗会导致蛋白质浪费。为了确定这种疗法是否影响吸收后状态的亮氨酸氧化和膳食氨基酸的处理，对八名正常受试者进行了两次随机研究，一次是在服用泼尼松（20毫克，每天三次）5天后，第二次是在不服用泼尼松的情况下作为对照。在吸收后状态，泼尼松治疗增加了亮氨酸、α-酮异己酸、葡萄糖、胰岛素和C肽的血浆浓度，以及通过同位素稀释技术和[1-13C]亮氨酸计算的亮氨酸碳通量和氧化。在输注化学成分确定的膳食期间，两个研究日的总亮氨酸碳通量和氧化增加相似，但泼尼松治疗期间亮氨酸氧化更大（P < 0.01）；尽管血浆胰岛素浓度较高（P < 0.05），但在对照日净亮氨酸平衡变为正值，但在泼尼松研究日仍保持负值或为零。这些研究表明，高剂量糖皮质激素治疗会损害人体吸收后和吸收状态下必需氨基酸亮氨酸的平衡。
High-dose glucocorticoid treatment results in protein wasting. To determine whether such therapy affects leucine oxidation in the postabsorptive state and the disposal of dietary amino acids, eight normal subjects were studied twice in random order, once after 5 days of prednisone (20 mg three times daily) and on a second occasion without prednisone as a control. In the postabsorptive state prednisone therapy increased (P < 0.05) plasma concentrations of leucine, .alpha.-ketoisocaproate, glucose, insulin, and C-peptide, as well as leucine carbon flux and oxidation calculated by means of isotope dilution techniques and [1-13C]leucine. During infusion of a chemically defined meal, total leucine carbon flux and oxidation increased similarly on both study days, but leucine oxidation was greater (P < 0.01) during prednisone treatment; net leucine balance became positive on the control day but remained negative or zero on the prednisone study day despite higher (P < 0.05) plasma insulin concentrations. These studies demonstrate that high-dose glucocorticoid treatment impairs the balance of the essential amino acid leucine in both the postabsorptive and absorptive states in humans.