The Min Oscillator Defines Sites of Asymmetric Cell Division in Cyanobacteria during Stress Recovery.
The Min Oscillator Defines Sites of Asymmetric Cell Division in Cyanobacteria during Stress Recovery.
复制标题
最小振荡器定义了蓝藻在应激恢复过程中不对称细胞分裂的位点。
DOI：
10.1016/j.cels.2018.10.006
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发表时间：
2018
期刊：
影响因子：
9.3
通讯作者：
Rust,MichaelJ
中科院分区：
文献类型：
--
作者：
Liao,Yi;Rust,MichaelJ
当资源充足时，许多杆状细菌通过精确、对称的分裂繁殖。然而，现实环境需要在限制性和宽松性增长条件之间波动。在这里，我们使用延时显微镜来研究蓝藻长聚球菌的分裂随光照强度的变化。我们发现昏暗条件下产生细长的细胞，其分裂遵循一个简单的规则：短于~ 8 μm的细胞对称分裂，但超过这个长度的细胞分裂变得不对称，通常产生短的~ 3 μm子细胞。我们证明了这种分裂策略是由Min系统实现的，它在较长的细胞中产生多节点模式和行波，有利于产生较短的子细胞。数学模型揭示了产生振荡最小模式的反馈回路是实现这些广义细胞分裂规则所必需的。因此，在短细胞中强制对称分裂的Min系统强烈抑制中细胞分裂。伸长细胞长。
When resources are abundant, many rod-shaped bacteria reproduce through precise, symmetric divisions. However, realistic environments entail fluctuations between restrictive and permissive growth conditions. Here, we use time-lapse microscopy to study the division of the cyanobacteriumSynechococcus elongatusas illumination intensity varies. We find that dim conditions produce elongated cells whose divisions follow a simple rule: cells shorter than ∼8 μm divide symmetrically, but above this length divisions become asymmetric, typically producing a short ∼3-μm daughter. We show that this division strategy is implemented by the Min system, which generates multi-node patterns and traveling waves in longer cells that favor the production of a short daughter. Mathematical modeling reveals that the feedback loops that create oscillatory Min patterns are needed to implement these generalized cell division rules. Thus, the Min system, which enforces symmetric divisions in short cells, acts to strongly suppress mid-cell divisions whenS. elongatuscells are long.