Effects of transforming growth factor-beta on human fetal adrenal steroid production.
Effects of transforming growth factor-beta on human fetal adrenal steroid production.
复制标题
转化生长因子-β 对人胎儿肾上腺类固醇产生的影响。
DOI：
10.1016/0303-7207(94)90002-7
复制
发表时间：
1994
影响因子：
4.1
通讯作者：
ParkerJr,CR
中科院分区：
文献类型：
--
作者：
Stankovic,AK;Dion,LD;ParkerJr,CR
转化生长因子-β（TGF-β）可抑制培养的人胎儿肾上腺细胞基础和促肾上腺皮质激素（ACTH）刺激的类固醇分泌。TGF-β的抑制作用具有时间和剂量依赖性。通常仅在≥ 0.1 ng TGF-β/ml治疗3天或更长时间后才观察到基础硫酸脱氢表雄酮（DS）产生的抑制。1 ng/ml TGF-β对ACTH刺激的DS产生的抑制作用比对胎儿区和新皮质细胞产生皮质醇的抑制作用更显著。还发现TGF-β干扰胎带细胞对毛喉素和二丁酰cAMP的反应产生DS和皮质醇。TGF-β干扰ACTH对胎儿区和新皮质细胞中细胞色素P45017αmRNA的刺激。这些结果表明，TGF-β差异抑制DS和皮质醇的生产由人胎儿肾上腺细胞和TGF-β对类固醇生成的作用的网站可能是远端的cAMP的产生。这些结果与其他结果沿着提示TGF-β可能在人肾上腺中起自分泌/旁分泌作用。
Transforming growth factor-β (TGF-β) was found to inhibit basal and ACTH-stimulated steroid production by cultured human fetal adrenal cells. The inhibitory effects of TGF-β were both time and dose-dependent. Inhibition of basal dehydroepiandrosterone sulfate (DS) production usually was noted only after 3 or more days of treatment with ≥ 0.1 ng TGF-β/ml. The inhibitory effects of l ng/ml TGF-β on ACTH-stimulated DS production were more striking than those on cortisol production by both fetal zone and neocortical cells. TGF-β also was found to interfere with DS and cortisol production by fetal zone cells in response to forskolin and dibutyryl cAMP. TGF-β interfered with ACTH stimulation of cytochrome P45017αmRNA in fetal zone and neocortex cells. These results are suggestive that TGF-β differentially inhibits DS and cortisol production by human fetal adrenal cells and that the site of TGF-β action on steroidogenesis may be distal to the generation of cAMP. Such results, along with those of others, are suggestive that TGF-β may play an autocrine/paracrine role in the human adrenal.