Development of a semi-defined medium supporting production of platensimycin and platencin by Streptomyces platensis
Development of a semi-defined medium supporting production of platensimycin and platencin by Streptomyces platensis
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DOI：
10.1038/ja.2012.97
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发表时间：
2013-02-01
影响因子：
3.3
通讯作者：
Demain, Arnold L.
中科院分区：
文献类型：
--
作者：
Aluotto, Sabrina;Tynan, Heather;Demain, Arnold L.
铂霉素和铂霉素是在默克研究实验室发现的化合物，并显示出有希望的抗菌活性。它们都是由放线菌platptomyces发酵产生的。默克公司报告了一种粗制的、不溶性的生产培养基来生产抗生素。为了测试不同的初级代谢物和无机化合物对这些抗生素生产的可能影响，需要一种化学定义的培养基。这些化合物对抗生素生产的影响可以为抗生素的前体和生物合成途径提供信息。我们已经测试和开发了许多不同程度的化学定义和溶解度的介质，以默克介质为起点。我们最新的生产介质，PM5，是可溶性和半定义。通过琼脂扩散试验、生物自显影法和高效液相色谱法，可以得到合适的化合物。抗生素主要存在于细胞外菌液中，而不在菌丝中。The Journal of Antibiotics (2013) 66,51 -54；doi: 10.1038 / ja.2012.97;2012年11月28日在线发布
Platensimycin and platencin are compounds that were discovered at Merck Research Laboratories and have shown promising antibacterial activity. They are both produced in fermentation by the actinomycete Streptomyces platensis. Merck reported a crude, insoluble production medium to produce the antibiotics. To test the possible effects of different primary metabolites and inorganic compounds on the production of these antibiotics, a chemically-defined medium is needed. The effects that these compounds have on production could provide information about the precursors and biosynthetic pathway of the antibiotics. We have tested and developed a number of media with varying degrees of chemical definition and solubility using the Merck medium as our starting point. Our latest production medium, PM5, is soluble and semi-defined. It yields suitable production of the compounds, as shown by agar diffusion assays, bioautography and HPLC. The antibiotics were located in the extracellular broths and not in the mycelia. The Journal of Antibiotics (2013) 66, 51-54; doi:10.1038/ja.2012.97; published online 28 November 2012