Cloning of inversion breakpoints in the Anopheles gambiae complex traces a transposable element at the inversion junction
Cloning of inversion breakpoints in the Anopheles gambiae complex traces a transposable element at the inversion junction
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DOI：
10.1073/pnas.95.21.12444
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发表时间：
1998-10-13
影响因子：
11.1
通讯作者：
Coluzzi, M
中科院分区：
文献类型：
--
作者：
Mathiopoulos, KD;Della Torre, A;Coluzzi, M
阿拉伯按蚊是冈比亚按蚊复合体的两个最有效的疟疾媒介之一，其特点是存在染色体偏着丝粒倒位。阐明这些倒位的性质和动态对于了解这种蚊子的种群遗传学和进化生物学以及对疟疾流行病学的影响至关重要。本文报道了阿拉比星自然发生的多态倒位2‘的断裂点的克隆。细胞学定位在断裂点近端的一个cdna克隆是分离跨越断裂点的粘粒克隆的起始材料。对周围序列的分析表明，在远端断裂点附近存在一个重复元件，该元件在不同的阿拉伯曲霉菌株中表现出不同的分布。对该区域的测序分析揭示了转座元件末端重复序列的元件特征。我们把这种假定的可转座元素称为奥德修斯。在自然发生的倒位2‘的交界处出现了奥德修斯，这表明倒位可能是转座元件活动的结果。奥德修斯终端区的特征及其在不同品系中的细胞学分布可能表明奥德修斯是相对较新的活动。
Anopheles arabiensis, one of the two most potent malaria vectors of the gambiae complex, is characterized by the presence of chromosomal paracentric inversions. Elucidation of the nature and the dynamics of these inversions is of paramount importance for the understanding of the population genetics and evolutionary biology of this mosquito and of the impact on malaria epidemiology. We report here the cloning of the breakpoints of the naturally occurring polymorphic inversion 2Rd' of A. arabiensis. A cDNA clone that cytologically mapped on the proximal breakpoint was the starting material for the isolation of a cosmid clone that spanned the breakpoint. Analysis of the surrounding sequences demonstrated that adjacent to the distal breakpoint lies a repetitive element that exhibits distinct distribution in different A. arabiensis strains. Sequencing analysis of that area revealed elements characteristic of transposable element terminal repeats. We called this presumed transposable element Odysseus. The presence of Odysseus at the junction of the naturally occuring inversion 2Rd' suggests that the inversion may be the result of the transposable element's activity. Characteristics of Odysseus' terminal region as well as its cytological distribution in different strains may indicate a relatively recent activity of Odysseus.