CD34+blood cells accelerate vascularization and healing of diabetic mouse skin wounds
CD34+blood cells accelerate vascularization and healing of diabetic mouse skin wounds
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DOI：
10.1159/000072701
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发表时间：
2003-07-01
影响因子：
1.7
通讯作者：
Schatteman, GC
中科院分区：
文献类型：
--
作者：
Sivan-Loukianova, E;Awad, OA;Schatteman, GC
糖尿病的特点是循环不良和血管生成受损，这似乎是糖尿病患者常见的皮肤病变频繁和伤口愈合不良的原因之一。改善血液循环的治疗通常可以改善糖尿病患者的伤口愈合。给予循环中的CD34+细胞，即可以作为内皮细胞前体的细胞，可以加速糖尿病小鼠缺血肢体的血流恢复。我们研究了这些细胞在低胰岛素血症(链脲佐菌素治疗)糖尿病小鼠全层皮肤创面加速血管重建和愈合的潜力。给创面注射人CD34+或CD34-外周血单核细胞或不注射人CD34+或CD34-外周血单个核细胞，并在此后的不同时间分析血管和愈合情况。与对照组相比，CD34+富集型细胞治疗可使创面面积缩短4天，加速表皮愈合，并迅速显著地加速创面血管重建。最初，血管形成的增加主要是通过血管直径的增加来调节的，但后来，观察到血管大小和数量的增加。这些发现表明，血源性祖细胞可能在治疗糖尿病皮肤病变方面具有治疗潜力，并为骨髓细胞如何在新生血管中发挥作用提供了深入的见解。版权所有(C)2003 S.Karger AG，巴塞尔。
Diabetes is characterized by poor circulation and impaired angiogenesis, which appear to contribute to the frequent skin lesions and poor wound healing common in diabetic patients. Therapies to improve circulation commonly improve wound healing in diabetic patients. Administration of circulating CD34+ cells, cells that can function as endothelial cell progenitors, accelerates blood flow restoration to ischemic limbs of diabetic mice. We have investigated the potential of these cells to accelerate revascularization and healing in full-thickness skin wounds of hypoinsulinemic (streptozotocin-treated) diabetic mice. Wounds were injected with human CD34+ or CD34- peripheral blood mononuclear cells or no cells, and analyzed for vascularity and healing at various times thereafter. Treatment with CD34+ enriched cells decreased wound size by 4 days after treatment, accelerated epidermal healing, and rapidly and dramatically accelerated revascularization of the wounds compared to controls. Initially increased vascularization was mediated principally by an increase in vessel diameter, but later, both an increase in vascular size and number were observed. These findings indicate that blood-derived progenitors may have therapeutic potential in the treatment of skin lesions in the setting of diabetes, and give insights into how bone marrow cells exert their effects on neovascularization. Copyright (C) 2003 S. Karger AG, Basel.