Involvement of glial cells in the nociceptive behaviors induced by a high-dose of histamine administered intrathecally.
Involvement of glial cells in the nociceptive behaviors induced by a high-dose of histamine administered intrathecally.
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神经胶质细胞参与鞘内施用高剂量组胺诱导的伤害性行为。
DOI：
10.1016/j.ejphar.2010.10.096
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发表时间：
2011
影响因子：
5
通讯作者：
S. Sakurada
中科院分区：
文献类型：
--
作者：
H. Mizoguchi;T. Komatsu;Yoko Iwata;C. Watanabe;Hiroyuki Watanabe;Tohru Orito;S. Katsuyama;A. Yonezawa;K. Onodera;T. Sakurada;S. Sakurada
用1600 pmol组胺诱发小鼠脊髓神经胶质细胞的伤害性行为。鞘内注射组胺（i. t.）产生伤害性行为，包括抓、咬和舔。i.t.能显著抑制组胺引起的伤害性行为。用神经胶质细胞抑制剂DL-氟柠檬酸或米诺环素预处理。在使用腰脊髓的蛋白质印迹分析中，i.t.组胺处理增加了N-甲基-D-天冬氨酸（NMDA）受体NR 1亚单位的磷酸化。组胺引起的NMDA受体NR 1亚基磷酸化的增加被i.t.用DL-氟柠檬酸或米诺环素预处理。我们以前曾报道过，1600 pmol组胺引起的伤害性行为被i.t.共同施用（5 R，10 S）-（+）-5-甲基-10，11-二氢-5H-二苯并[a，d]环庚烯-5，10-亚胺（MK-801）（一种NMDA受体的离子通道阻断剂）或胍丁胺（一种NMDA受体NR 1亚基上的多胺识别位点的拮抗剂）。在本研究中，增加磷酸化的NMDA受体的NR 1亚基组胺也被取消了i.t.联合给予胍丁胺或MK-801。结果提示，1600 pmol组胺通过刺激脊髓神经胶质细胞NMDA受体NR 1亚单位上的多胺识别位点而发挥伤害性行为。
The involvement of spinal glial cells in the nociceptive behaviors induced by 1600pmol of histamine was determined in mice. Histamine injected intrathecally (i.t.) produced nociceptive behaviors, consisting of scratching, biting and licking. The nociceptive behaviors induced by histamine were significantly suppressed by i.t. pretreatment with the glial cell inhibitor DL-fluorocitric acid or minocycline. In Western blot analysis using lumber spinal cords, i.t. treatment with histamine increased the phosphorylation of the NR1 subunit of N-methyl-D-aspartate (NMDA) receptors. The increased phosphorylation of the NR1 subunit of NMDA receptors by histamine was abolished by i.t. pretreatment with DL-fluorocitric acid or minocycline. We have previously reported that the nociceptive behaviors induced by 1600pmol of histamine were significantly suppressed by the i.t. co-administration of (5R,10S)-(+)-5-methyl-10,11-dihydro-5H-dibenzo[a,d]cycloheptene-5,10-imine (MK-801), an ion channel blocker of NMDA receptors, or agmatine, an antagonist for the polyamine recognition site on the NR1 subunit of NMDA receptors. In the present study, the increased phosphorylation of the NR1 subunit of NMDA receptors by histamine was also abolished by i.t. co-administration of agmatine or MK-801. The present results suggest that histamine at 1600pmol elicits nociceptive behaviors by stimulating the polyamine recognition site on the NR1 subunit of NMDA receptors on spinal glial cells.