Crag regulates epithelial architecture and polarized deposition of basement membrane proteins in Drosophila
Crag regulates epithelial architecture and polarized deposition of basement membrane proteins in Drosophila
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DOI：
10.1016/j.devcel.2007.12.012
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发表时间：
2008-03-01
影响因子：
11.8
通讯作者：
Schupbach, Trudi
中科院分区：
文献类型：
--
作者：
Denef, Natalie;Chen, Yu;Schupbach, Trudi
上皮的极化结构依赖于细胞骨架、运输机制、细胞-细胞和细胞-基质粘附之间的相互作用。具体来说，与基底膜（BM）的接触对细胞极性很重要，基底膜是上皮基底面的细胞外基质。然而，人们对BM蛋白本身如何实现极化分布知之甚少。在果蝇滤泡上皮的遗传筛选中，我们发现了Crag的突变，该突变编码一种保守蛋白，其结构域与膜运输有关。卵泡细胞突变的克雷格失去上皮完整性，经常成为侵入性的。Crag的缺失导致上皮细胞两侧BM组分的异常积聚，而不直接影响顶端或基底外侧膜蛋白的分布。这种缺陷通常不会在影响上皮完整性的突变体中观察到。我们认为Crag通过调节BM蛋白的极化分泌，在组织上皮结构中起着独特的作用。
The polarized architecture of epithelia relies on an interplay between the cytoskeleton, the trafficking machinery, and cell-cell and cell-matrix adhesion. Specifically, contact with the basement membrane (BM), an extracellular matrix underlying the basal side of epithelia, is important for cell polarity. However, little is known about how BM proteins themselves achieve a polarized distribution. In a genetic screen in the Drosophila follicular epithelium, we identified mutations in Crag, which encodes a conserved protein with domains implicated in membrane trafficking. Follicle cells mutant for Crag lose epithelial integrity and frequently become invasive. The loss of Crag leads to the anomalous accumulation of BM components on both sides of epithelial cells without directly affecting the distribution of apical or basolateral membrane proteins. This defect is not generally observed in mutants affecting epithelial integrity. We propose that Crag plays a unique role in organizing epithelial architecture by regulating the polarized secretion of BM proteins.