Ion Mobility Spectrometry: Fundamental Concepts, Instrumentation, Applications, and the Road Ahead
Ion Mobility Spectrometry: Fundamental Concepts, Instrumentation, Applications, and the Road Ahead
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DOI：
10.1007/s13361-019-02288-2
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发表时间：
2019-11-01
影响因子：
3.2
通讯作者：
Baker, Erin S.
中科院分区：
文献类型：
--
作者：
Dodds, James N.;Baker, Erin S.
离子迁移谱（IMS）是一种快速分离技术，作为一个研究领域已经经历了指数增长。IMS与质谱（IMS-MS）的接口提供了额外的分析能力，因为每种技术的互补分离能够对检测到的分析物进行多维表征。IMS分离发生在毫秒级的时间尺度上，因此可以很容易地嵌套到传统的GC和LC/MS工作流程中。然而，新的IMS方法的不断发展已经产生了一定程度的混乱，关于每一个的优点和缺点。在这个关键的见解，我们的目标是澄清一些常见的误解，为新用户在社区有关的基本概念的各种IMS工具平台（即，DTIMS、TWIMS、TIMS、FAIMS和DMA），同时解决与每一个相关联的优点和缺点。常见的IMS-MS应用也讨论了在这篇评论中，如分离异构物种，执行信号过滤MS，并将碰撞截面（CCS）值到目标和非目标的omics为基础的工作流程作为额外的离子描述符化学注释。虽然在以常规方式收集移动性信息之前，IMS社区必须解决许多挑战，但未来充满了可能性。
Ion mobility spectrometry (IMS) is a rapid separation technique that has experienced exponential growth as a field of study. Interfacing IMS with mass spectrometry (IMS-MS) provides additional analytical power as complementary separations from each technique enable multidimensional characterization of detected analytes. IMS separations occur on a millisecond timescale, and therefore can be readily nested into traditional GC and LC/MS workflows. However, the continual development of novel IMS methods has generated some level of confusion regarding the advantages and disadvantages of each. In this critical insight, we aim to clarify some common misconceptions for new users in the community pertaining to the fundamental concepts of the various IMS instrumental platforms (i.e., DTIMS, TWIMS, TIMS, FAIMS, and DMA), while addressing the strengths and shortcomings associated with each. Common IMS-MS applications are also discussed in this review, such as separating isomeric species, performing signal filtering for MS, and incorporating collision cross-section (CCS) values into both targeted and untargeted omics-based workflows as additional ion descriptors for chemical annotation. Although many challenges must be addressed by the IMS community before mobility information is collected in a routine fashion, the future is bright with possibilities.