DETERMINATION OF CILIARY POLARITY PRECEDES DIFFERENTIATION IN THE EPITHELIAL-CELLS OF QUAIL OVIDUCT
DETERMINATION OF CILIARY POLARITY PRECEDES DIFFERENTIATION IN THE EPITHELIAL-CELLS OF QUAIL OVIDUCT
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DOI：
10.1016/0248-4900(91)90072-u
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发表时间：
1991-01-01
影响因子：
2.7
通讯作者：
ALLART, JP
中科院分区：
文献类型：
--
作者：
BOISVIEUXULRICH, E;SANDOZ, D;ALLART, JP
在鹌鹑输卵管上皮，在所有后生动物和原生动物纤毛细胞，纤毛跳动在一个协调的周期。它们排列成与输卵管前后轴方向一致的极化模式，很可能负责将卵子和卵白色蛋白从漏斗部运输到子宫。睫状体搏动的方向与基底体的方向有关，由外侧基底足的位置指示，其指向睫状体搏动的主动行程的方向。睫状皮质的这种排列是纤毛发生和输卵管发育的最终步骤。纤毛首先以随机方向发育，随后重新定向，与皮质细胞骨架的发育同时进行。为了了解在输卵管发育过程中，基体和纤毛的最终方向是何时确定的，我们对一段未成熟的输卵管进行了显微手术逆转。然后，在毛诱导的发展和纤毛发生，纤毛的方向进行了检查，在倒置段和正常部分的纤毛上皮。在倒置的部分，方向是相反的，如所示的视频记录的有效流动的方向产生的殴打纤毛，由三维弯曲形式的纤毛固定在殴打周期和扫描电子显微镜筛选，并通过透射电子显微镜在薄切片中看到的位置的基体附属物。这些结果表明，基体和纤毛的方向是不可逆的确定之前，由内源性信号存在于早期的未成熟输卵管的细胞，在增殖阶段传递到子细胞，并在纤毛发生结束时表达的发展。
In quail oviduct epithelium, as in all metazoan and protozoan ciliated cells, cilia beat in a coordinated cycle. They are arranged in a polarized pattern oriented according to the anteroposterior axis of the oviduct and are most likely responsible for transport of the ovum and egg white proteins from the infundibulum toward the uterus. Orientation of ciliary beating is related to that of the basal bodies, indicated by the location of the lateral basal foot, which points in the direction of the active stroke of ciliary beating. This arrangement of the ciliary cortex occurs as the ultimate step in ciliogenesis and following the oviduct development. Cilia first develop in a random orientation and reorient later, simultaneously with the development of the cortical cytoskeleton. In order to know when the final orientation of basal bodies and cilia is determined in the course of oviduct development, microsurgical reversal of a segment of the immature oviduct was performed. Then, after hormone-induced development and ciliogenesis, ciliary orientation was examined in the inverted segment and in normal parts of the ciliated epithelium. In the inverted segment, orientation was reversed, as shown by a video recording of the direction of effective flow produced by beating cilia, by the three-dimensional bending forms of cilia immobilized during the beating cycle and screened by scanning electron microscopy, and by the position of basal body appendages as seen in thin sections by transmission electron microscopy. These results demonstrate that basal body and ciliary orientation are irreversibly determined prior to development by an endogenous signal present early in the cells of the immature oviduct, transmitted to daughter cells during the proliferative phase and expressed at the end of ciliogenesis.