The ELONGATED gene of Arabidopsis acts independently of light and gibberellins in the control of elongation growth
The ELONGATED gene of Arabidopsis acts independently of light and gibberellins in the control of elongation growth
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DOI：
10.1046/j.1365-313x.1996.09030305.x
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发表时间：
1996-03-01
期刊：
影响因子：
7.2
通讯作者：
Koornneef, M
中科院分区：
文献类型：
--
作者：
Halliday, K;Devlin, PF;Koornneef, M
从EMS诱变的拟南芥ga 4突变体群体中分离出一种新的伸长突变体。在与兰茨贝格直立（Ler）野生型（WT）回交之后，随后自交，在GA 4背景中鉴定突变体表型。该突变体的幼苗被命名为elg（elongated），其特征是下胚轴和叶柄伸长，叶窄，有点上倾，开花早。hy 1-hy 5突变体的等位性测试表明elg与这些长下胚轴突变体中的任何一个都不是等位的。从连锁分析，elg的定位在4号染色体上，cer 2和ap 2之间已经建立。elg幼苗的多效性表型暗示光敏色素和/或赤霉素（GA）功能的破坏。虽然elg突变体显示出光依赖性长下胚轴表型，elg幼苗保留了全方位的光形态发生反应和elg突变与光形态发生突变体phyB，hy 1和hy 2的相加作用。这表明ELG的作用独立于光敏色素的作用。elg突变部分抑制了GA缺乏对伸长生长的影响，并且，虽然elg半乳糖幼苗比半乳糖幼苗更伸长，但两种基因型以相同的方式响应于施加的GA。施加的GA和elg突变相互作用加性表明，ELG作用独立于GA作用。
A novel elongated mutant has been isolated from EMS-mutagenized populations of the Arabidopsis thaliana ga4 mutant. After backcrossing with the Landsberg erecta (Ler) wild-type (WT) followed by selfing, the mutant phenotype was identified in the GA4 background. Seedlings of the mutant, which has been named elg(elongated), are characterized by elongated hypocotyls and petioles, leaves that are narrow and somewhat epinastic and early flowering. Allelism tests with the hy1-hy5 mutants indicate that elg is not allelic with any of these long-hypocotyl mutants. From linkage analyses, the location of elg on chromosome 4, between cer2 and ap2 has been established. The pleiotropic phenotype of elg seedlings is suggestive of a disruption of phytochrome and/or gibberellin (GA) function. Although the elg mutant displays a light-dependent long-hypocotyl phenotype, elg seedlings retain a full range of photomorphogenic responses and the elg mutation acts additively with the photomorphogenic mutants phyB, hy1 and hy2. This suggests that ELG acts independently of phytochrome action. The elg mutation partially suppresses the effect of GA-deficiency on elongation growth, and, although elg gal seedlings are more elongated than gal seedlings, both genotypes respond in the same way to applied GA, That applied GA and the elg mutation interact additively suggests that ELG acts independently of GA action.