Volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in WEHI 231 and RAJI B lymphoma cells and splenic B cells
Volatile fatty acid, metabolic by-product of periodontopathic bacteria, induces apoptosis in WEHI 231 and RAJI B lymphoma cells and splenic B cells
复制标题
DOI：
10.1128/iai.66.6.2587-2594.1998
复制
发表时间：
1998-06-01
影响因子：
3.1
通讯作者：
Fukushima, K
中科院分区：
文献类型：
--
作者：
Kurita-Ochiai, T;Ochiai, K;Fukushima, K
检测了丁酸（牙周病细菌的胞外代谢物）诱导小鼠WEHI 231细胞、脾B细胞和人RAJI细胞凋亡的能力。牙龈卟啉单胞菌、洛氏普雷沃菌和具核梭杆菌的培养滤液含有高百分比的丁酸，可诱导WEHI 231细胞的DNA断裂。挥发性脂肪酸，特别是丁酸，显着抑制B细胞活力的浓度依赖性的方式。DNA片段化分析表明，丁酸在WEHI 231细胞（用1.25 mM丁酸处理后6 h）、脾B细胞（用1.25 mM丁酸）和RGJI细胞（用2.5 mM丁酸）中快速诱导细胞凋亡。WEHI 231细胞在丁酸作用16 h后，出现典型的DNA梯状断裂和染色质浓缩、亚二倍体核等凋亡改变。细胞周期分析表明丁酸可使细胞阻滞于G（1）期。抑制实验表明丁酸诱导的WEHI 231和脾B细胞凋亡可被钙调素抑制剂W-7抑制。这些结果表明，钙调素依赖性调节参与丁酸的信号转导途径。
The ability of butyric acid, an extracellular metabolite from periodontopathic bacteria, to induce apoptosis in murine WEHI 231 cells, splenic B cells, and human RAJI cells was examined. The culture filtrate of Porphyromonas gingivalis, Prevotella loescheii, and Fusobacterium nucleatum, which contains high a percentage of butyric acid, induced DNA fragmentation in WEHI 231 cells. Volatile fatty acid, especially butyric acid, significantly suppressed B-cell viability in a concentration-dependent fashion. The DNA fragmentation assay indicated that butyric acid rapidly induced apoptosis in WEHI 231 cells (with 1.25 mM butyric acid and 6 h after treatment), splenic B cells (with 1.25 mM butyric acid), and RGJI cells (with 2.5 mM butyric acid). Incubation of WEHI 231 cells with butyric acid for 16 h resulted in the typical ladder pattern of DNA fragmentation and the apoptoic change such as chromatin condensation and hypodiploid nuclei. Cell cycle analysis implied that butyric acid arrested the cells at the G(1) phase, The inhibitory assay suggested that butyric acid-induced apoptosis of WEHI 231 and splenic B cells was inhibited by W-7, a calmodulin inhibitor. These results suggest that calmodulin-dependent regulation is involved in the signal transduction pathway of butyric acid.