Human ClpP protease: CDNA sequence, tissue-specific expression and chromosomal assignment of the gene
Human ClpP protease: CDNA sequence, tissue-specific expression and chromosomal assignment of the gene
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DOI：
10.1016/0014-5793(95)01353-9
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发表时间：
1995-12-18
期刊：
影响因子：
3.5
通讯作者：
Gregersen, N
中科院分区：
文献类型：
--
作者：
Bross, P;Andresen, BS;Gregersen, N
我们通过筛选 EMBL 核苷酸数据库，鉴定了三个与大肠杆菌 ClpP 氨基酸序列具有显着同源性的重叠人类表达序列标签，利用该序列信息，我们应用 cDNA 末端的 5' 和 3' 快速扩增 (RACE) 来扩增和测序两个重叠片段中的人类 clpP cDNA，开放阅读框编码 277 个氨基酸长的前体多肽，存在围绕活性位点残基的两个 ClpP 特异性基序，并且与观察到来自其他生物体的 ClpP，Northern 印迹显示骨骼肌中 clpP mRNA 的相对表达水平较高，心脏、肝脏和胰腺中水平中等，脑、胎盘、肺和肾脏中水平较低。通过对人类/啮齿动物细胞杂交的分析，人类 clpP 基因被分配到 19 号染色体。
We identified three overlapping human expressed sequence tags with significant homology to the E. coli ClpP amino sequence by screening the EMBL nucleotide database, With this sequence information we applied 5' and 3'-rapid amplification of cDNA ends (RACE) to amplify and sequence human clpP cDNA in two overlapping fragments, The open reading frame encodes a 277 amino acid long precursor polypeptide, Two ClpP specific motifs surrounding the active site residues are present and extensive homology to ClpP's from other organisms was observed, Northern blotting showed high relative expression levels of clpP mRNA in skeletal muscle, intermediate levels in heart, liver and pancreas, and low levels in brain, placenta, lung and kidney, By analysis of human/rodent cell hybrids the human clpP gene was assigned to chromosome 19.