Chinese hamster lung cells synthesize and confine to the cellular domain a collagen composed solely of B chains.
Chinese hamster lung cells synthesize and confine to the cellular domain a collagen composed solely of B chains.
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中国仓鼠肺细胞合成仅由 B 链组成的胶原蛋白并将其限制在细胞域内。
DOI：
10.1073/pnas.77.9.5206
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发表时间：
1980
影响因子：
11.1
通讯作者：
Miller,EJ
中科院分区：
文献类型：
--
作者：
Haralson,MA;Mitchell,WM;Rhodes,RK;Kresina,TF;Gay,R;Miller,EJ
从培养的中国仓鼠肺（CHL）细胞层中提取酸溶性胶原蛋白，经有限胃蛋白酶消化和差异盐分离分离。变性条件下聚丙烯酰胺凝胶电泳结果显示，还原前后均存在明显分子量为12万道尔顿的胶原链，表明原生分子中不存在链间二硫键。在变性条件下cm -纤维素层析时，CHL细胞层胶原链的大部分（> 90%）作为相对基本的组分被洗脱，略早于人α 2(I)链，与人B链一致。此外，从人B链和CHL细胞层链中提取的溴化氰肽的cm -纤维素洗脱谱基本相同。用人B链亲和纯化抗体检测培养的CHL细胞，发现这种胶原蛋白定位在细胞周围的细胞外基质中。此外，对培养基的分析表明没有任何类似的胶原链。这些数据为仅由B链组成的胶原蛋白分子形式的存在提供了额外的证据，并表明这种胶原蛋白分子形式对该系统中的细胞层具有不同寻常的亲和力。
The acid-soluble collagen extracted from cultured Chinese hamster lung (CHL) cell layers has been isolated after limited pepsin digestion and differential salt fractionation. Polyacrylamide gel electrophoresis of this material under denaturing conditions showed the presence of collagen chains with an apparent molecular mass of 120,000 daltons both before and after reduction, indicating the absence of interchain disulfide bonds in the native molecule. When chromatographed on CM-cellulose under denaturing conditions, the majority (> 90%) of the CHL cell layer collagen chains eluted as relatively basic components slightly before the human alpha 2(I) chain and coincident with the human B chain. In addition, the CM-cellulose elution profiles of the cyanogen bromide peptides derived from the human B chain and from the CHL cell layer chain were essentially identical. Examination of CHL cells in culture by using affinity-purified antibody to human B chain revealed this collagen to be localized in an extracellular matrix surrounding the cells. Furthermore, analysis of the culture medium indicated the absence of any comparable collagen chain. These data provide additional evidence for the existence of a molecular form of collagen composed solely of B chains and suggest that this molecular form of collagen has an unusual affinity for the cell layer in this system.