A flash in the pan: dissecting dynamic amyloid intermediates using fluorescence.
A flash in the pan: dissecting dynamic amyloid intermediates using fluorescence.
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DOI：
10.1016/j.febslet.2013.02.044
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发表时间：
2013-04-17
期刊：
影响因子：
3.5
通讯作者：
Rhoades E
中科院分区：
文献类型：
--
作者：
Nath A;Rhoades E
几种广泛和严重的退行性疾病的特征在于淀粉样蛋白聚集体在受影响的组织中的沉积。虽然有很大的兴趣，在完整的描述所涉及的蛋白质的聚集途径，分子水平的理解是由自组装过程的复杂性阻碍。特别是，聚集的早期阶段，其中动态的，异质的，往往是有毒的中间体填充，是耐高分辨率的结构表征。荧光光谱法是一个强大的和通用的工具，这样的分析。在这篇综述中，我们调查它的应用，以提供三个选定的蛋白质：IAPP，α-突触核蛋白和tau蛋白的淀粉样蛋白中间状态的残基特异性信息。
Several widespread and severe degenerative diseases are characterized by the deposition of amyloid protein aggregates in affected tissues. While there is great interest in the complete description of the aggregation pathway of the proteins involved, a molecular level understanding is hindered by the complexity of the self-assembly process. In particular, the early stages of aggregation, where dynamic, heterogeneous and often toxic intermediates are populated, are resistant to high-resolution structural characterization. Fluorescence spectroscopy is a powerful and versatile tool for such analysis. In this review, we survey its application to provide residue-specific information about amyloid intermediate states for three selected proteins: IAPP, α-synuclein, and tau.