Feeding restriction alleviates high carbohydrate diet-induced oxidative stress and inflammation of Megalobrama amblycephala by activating the AMPK-SIRT1 pathway
Feeding restriction alleviates high carbohydrate diet-induced oxidative stress and inflammation of Megalobrama amblycephala by activating the AMPK-SIRT1 pathway
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饲喂限制通过激活 AMPK-SIRT1 通路减轻高碳水化合物饮食诱导的团头鲂氧化应激和炎症
DOI：
10.1016/j.fsi.2019.06.057
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发表时间：
2019
影响因子：
4.7
通讯作者：
Li Xiang Fei
中科院分区：
文献类型：
--
作者：
Xu Chao;Liu Wen Bin;Remo Sofie Charlotte;Wang Bing Ke;Shi Hua Juan;Zhang Li;Liu Jia Dai;Li Xiang Fei
研究了限饲对高碳水化合物饲料条件下团头鲂生长性能、氧化应激和炎症反应的影响。将体重为46.94 ± 0.04 g的鱼随机分为4组，分别喂饱对照饲料（30%碳水化合物）和HC饲料（43%碳水化合物）的3个饱足水平（100%（HC 1）、80%（HC 2）和60%（HC 3））8周。结果表明，HC 1组肉鸡的终重、增重率、比生长率、饲料转化率、肝脏总抗氧化能力、超氧化物歧化酶和过氧化氢酶活性、AMP/ATP比值、p-AMPKα/t-AMPKα比值、SIRT 1蛋白表达和AMPKα2、SIRT 1、核因子红细胞2相关因子2（Nrf 2）、过氧化氢酶（CAT）、锰超氧化物歧化酶（Mn-SOD）、谷胱甘肽过氧化物酶1（GPx 1）和白细胞介素10（IL 10）与对照组相比，而蛋白质效率比（PER）、氮保留效率（NRE）、能量保留效率（ERE）、血糖水平、谷丙转氨酶（AST）和谷草转氨酶（ALT）活性、肝脏中的丙二醛（MDA）含量、肿瘤坏死因子α（TNF α）和白细胞介素1β（IL 1β）、ATP和AMP含量以及Kelch样ECH相关蛋白1（Keap 1）、Ik B激酶α（IKK α）、核因子kappa B（NF-κB）、TNF α、IL 1β、白细胞介素6（IL 6）和转化生长因子β（TGF β）。HC组的SGR、PER、NRE、ERE、肝脏T-AOC、SOD和CAT活性、AMP/ATP比值、p-AMPKα/t-AMPKα比值、SIRT 1蛋白表达以及AMPKα2、Nrf 2、CAT、铜锌超氧化物歧化酶（Cu/Zn SOD）的肝转运量均高于HC组（Cu/Zn-SOD）、Mn-SOD、GPx 1、谷胱甘肽S-转移酶（GST）和白细胞介素10（IL 10）的含量，而肝脏IL 6含量和IKK α的转录则相反。总体而言，80%饱食通过激活AMPK-SIRT 1通路和上调Nrf 2调节的抗氧化酶的活性和转录，以及抑制NF-κ B介导的促炎细胞因子的水平和转录，改善了饲喂HC饲料的团头鲂的生长性能，减轻了氧化应激和炎症。
This study investigated the effects of restricted feeding on the growth performance, oxidative stress and inflammation ofMegalobrama amblycephalafed high-carbohydrate (HC) diets. Fish (46.94 ± 0.04 g) were randomly assigned to four groups containing the satiation of a control diet (30% carbohydrate) and three satiate levels (100% (HC1), 80% (HC2) and 60% (HC3)) of the HC diets (43% carbohydrate) for 8 weeks. Results showed that HC1 diet remarkably decreased final weight (FW), weight gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR), hepatic activities of total anti-oxidation capacity (T-AOC), superoxide dismutase (SOD) and catalase (CAT), the AMP/ATP ratio, the p-AMPKα/t-AMPKα ratio, sirtuin-1 (SIRT1) protein expression and hepatic transcriptions of AMPKα2, SIRT1, nuclear factor erythroid 2-related factor 2 (Nrf2), catalase (CAT), manganese superoxide dismutase (Mn-SOD), glutathione peroxidase 1 (GPx1) and interleukin10 (IL 10) compared to the control group, whereas the opposite was true for protein efficiency ratio (PER), nitrogen retention efficiency (NRE), energy retention efficiency (ERE), plasma glucose levels, alanine transaminase (AST) and aspartate aminotransferase (ALT) activities, hepatic contents of malondialdehyde (MDA), tumour necrosis factor α (TNF α) and interleukin 1β (IL 1β), ATP and AMP contents and hepatic transcriptions of kelch-like ECH associating protein 1 (Keap1), IkB kinase α (IKK α), nuclear factor kappa B (NF-κB), TNF α, IL 1β, interleukin 6 (IL 6) and transforming growth factor β (TGF β). As for the HC groups, fish fed the HC2 diet obtained relatively high values of SGR, PER, NRE, ERE, hepatic activities of T-AOC, SOD and CAT, the AMP/ATP ratio, the p-AMPKα/t-AMPKα ratio, SIRT1 protein expression and hepatic transcriptions of AMPKα2, Nrf2, CAT, copper/zinc superoxide dismutase (Cu/Zn-SOD), Mn-SOD, GPx1, glutathione S-transferase (GST) and interleukin10 (IL 10), while the opposite was true for hepatic content of IL 6 and transcription of IKK α. Overall, an 80% satiation improved the growth performance and alleviated the oxidative stress and inflammation of blunt snout bream fed HC diets via the activation of the AMPK-SIRT1 pathway and the up-regulation of the activities and transcriptions of Nrf2-modulated antioxidant enzymes coupled with the depression of the levels and transcriptions of the NF-κB-mediated pro-inflammatory cytokines.