DIFFERENCES IN CHLOROPHYLL-PROTEIN COMPLEXES AND COMPOSITION OF POLYPEPTIDES BETWEEN THYLAKOIDS FROM BUNDLE SHEATHS AND MESOPHYLL-CELLS IN MAIZE
DIFFERENCES IN CHLOROPHYLL-PROTEIN COMPLEXES AND COMPOSITION OF POLYPEPTIDES BETWEEN THYLAKOIDS FROM BUNDLE SHEATHS AND MESOPHYLL-CELLS IN MAIZE
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DOI：
10.1111/j.1432-1033.1985.tb08692.x
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发表时间：
1985-01-01
期刊：
影响因子：
--
通讯作者：
GHISI, R
中科院分区：
文献类型：
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作者：
BASSI, R;PERUFFO, AD;GHISI, R
通过管十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（SDS-PAGE）检查来自酶促分离的束鞘和叶肉组织叶绿体的类囊体的叶绿素蛋白。峰之间叶绿素的分布存在差异。叶绿素蛋白 a 峰 (CPa) 被许多作者认为是光系统 II (PSII) 反应中心，但在束鞘类囊体样品中不存在。平板 SDS-PAGE 显示仅具有 PSII 活性的叶绿体亚级分的 PSII 制剂中不存在多肽。这一发现证实了安德森关于基粒和基质类囊体结构的假设。
Thylakoids from enzymatically separated bundle sheath and mesophyll tissue chloroplast were examined for their chlorophyll-proteins by tube sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS-PAGE). Differences were found in distribution of chlorophyll among peaks. The chlorophyll-protein a peak (CPa), considered to be the photosystem II (PSII) reaction center by many authors, was absent in bundle sheath thylakoid samples. The slab SDS-PAGE revealed the absence of the polypeptide present in PSII preparations of chloroplast subfractions having only PSII activity. This finding confirms Anderson''s hypothesis of the structure of grana and stroma thylakoids.