Diversity of methanogens and sulfate-reducing bacteria in the interfaces of five deep-sea anoxic brines of the Red Sea
Diversity of methanogens and sulfate-reducing bacteria in the interfaces of five deep-sea anoxic brines of the Red Sea
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DOI：
10.1016/j.resmic.2015.07.002
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发表时间：
2015-11-01
影响因子：
2.6
通讯作者：
Stingl, Ulrich
中科院分区：
文献类型：
--
作者：
Guan, Yue;Hikmawan, Tyas;Stingl, Ulrich
海洋深层高盐缺氧盆地（DHAB）的特点是从上覆海水到卤水体的过渡过程中物理化学条件发生剧烈变化。地中海多个 DHAB 的盐水-海水界面 (BSI) 已被证明具有产甲烷和硫酸盐还原活性，但尚未进行系统研究来解决红海 DHAB 中产甲烷和硫酸盐还原群落的潜在功能多样性。在这里，我们使用定量 PCR 评估了细菌和古细菌的相对丰度，并对几乎全长的 16S rRNA 基因以及编码甲基辅酶 M 还原酶 (mcrA) 和异化亚硫酸盐还原酶 (dsrA) α 亚基的功能标记基因进行了系统发育分析。在大多数地点，细菌比古细菌占主导地位，其中大多数属于三角洲变形菌门，而奇古菌是所有采样地点中最普遍的古细菌。亚特兰蒂斯二号深渊的上部对流层承受着日益恶劣的环境条件，主要由热原体纲（海洋底栖 E 组和地中海卤水湖 1 组）成员主导。我们的研究揭示了所有五个研究地点独特的微生物组成、特定生态位群体的存在，以及与产甲烷菌群落相比，硫酸盐还原菌群总体上具有更高的多样性。 (C) 2015 年巴斯德研究所。由 Elsevier Masson SAS 出版。版权所有。
Oceanic deep hypersaline anoxic basins (DHABs) are characterized by drastic changes in physico-chemical conditions in the transition from overlaying seawater to brine body. Brine-seawater interfaces (BSIs) of several DHABs across the Mediterranean Sea have been shown to possess methanogenic and sulfate-reducing activities, yet no systematic studies have been conducted to address the potential functional diversity of methanogenic and sulfate-reducing communities in the Red Sea DHABs. Here, we evaluated the relative abundance of Bacteria and Archaea using quantitative PCR and conducted phylogenetic analyses of nearly full-length 16S rRNA genes as well as functional marker genes encoding the alpha subunits of methyl-coenzyme M reductase (mcrA) and dissimilatory sulfite reductase (dsrA). Bacteria predominated over Archaea in most locations, the majority of which were affiliated with Deltaproteobacteria, while Thaumarchaeota were the most prevalent Archaea in all sampled locations. The upper convective layers of Atlantis II Deep, which bear increasingly harsh environmental conditions, were dominated by members of the class Thermoplasmata (Marine Benthic Group E and Mediterranean Sea Brine Lakes Group 1). Our study revealed unique microbial compositions, the presence of niche-specific groups, and collectively, a higher diversity of sulfate-reducing communities compared to methanogenic communities in all five studied locations. (C) 2015 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.