High Performance, Multiplexed Lung Cancer Biomarker Detection on a Plasmonic Gold Chip
High Performance, Multiplexed Lung Cancer Biomarker Detection on a Plasmonic Gold Chip
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等离激元金芯片上的高性能、多重肺癌生物标志物检测
DOI：
10.1002/adfm.201603547
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发表时间：
2016-11-22
影响因子：
19
通讯作者：
Qian, Kun
中科院分区：
文献类型：
--
作者：
Liu, Bin;Li, Yaling;Qian, Kun
肺癌的诊断是使用等离子体金（pGOLD）芯片通过对患者血清样品中的癌胚抗原（CEA）、Cyfra 21 -1和神经元特异性烯醇化酶（NSE）进行多路近红外（NIR）检测来进行的。与传统的生物芯片和目前在医院中使用的Luminex技术相比，在pGOLD芯片上对10 μ L人血清或全血样品中的CEA、Cyfra 21 -1和NSE进行的多重微阵列分析具有近50倍的增强，导致显著改善的定量限、检测限、再现性和更高的诊断灵敏度和特异性。
Diagnosis of lung cancer is performed using a plasmonic gold (pGOLD) chip through multiplexed near-infrared (NIR) detection of carcino-embryonic antigen (CEA), Cyfra21-1, and neuron-specific enolase (NSE) in the serum samples of patients. With approximate to 50-fold enhancement of NIR fluorescence, multiplexed microarray analysis of CEA, Cyfra21-1, and NSE in 10 mu L of human serum or whole blood samples on pGOLD chip leads to markedly improved limit-of-quantification, limit-of-detection, reproducibility, and higher diagnostic sensitivity and specificity compared to traditional biochips and Luminex technology currently in use in hospitals.