Lamellipodia in invasion
Lamellipodia in invasion
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DOI：
10.1006/scbi.2000.0363
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发表时间：
2001-04-01
影响因子：
14.5
通讯作者：
Segall, JE
中科院分区：
文献类型：
--
作者：
Condeelis, JS;Wyckoff, JB;Segall, JE
GFP标记的转移性肿瘤细胞的体内成像揭示了细胞朝向血管的方向。在对配体如EGF的趋化反应期间，肿瘤细胞的定向始于片足延伸。对片足延伸中某些下游事件的评估表明：（1）其他EGF受体的质膜分布是均匀的，但内化的受体聚集在离EGF来源最近的细胞一侧;（2）PI-3激酶的α p110亚型是必需的;（3）片足的突出依赖于Arp 2/3复合物和cofilin的联合作用以产生丝状肌动蛋白。
In vivo imaging of GFP-labeled metastatic tumor cells reveals cell orientation towards blood vessels. Orientation of tumor cells during chemotactic responses to ligands such as EGF begins with lamellipod extension. Evaluation of some of the downstream events in lamellipod extension indicates: (1) plasma membrane distribution other EGF receptor is uniform bur internalized receptor accumulates on the side of the cell closest to the source of EGF; (2) the alpha p110 isoform of PI-3 kinase is required; ann (3) protrusion of the lamellipod relies upon the combined actions of the Arp2/3 complex and cofilin for generation of filamentous actin.