Analysis of Streptococcus salivarius urease expression using continuous chemostat culture.
Analysis of Streptococcus salivarius urease expression using continuous chemostat culture.
复制标题
使用连续恒化培养分析唾液链球菌脲酶表达。
DOI：
10.1111/j.1574-6968.1996.tb07993.x
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发表时间：
1996
影响因子：
2.1
通讯作者：
Burne,RA
中科院分区：
文献类型：
--
作者：
Chen,YY;Burne,RA
口腔中细菌脲酶从尿素产生碱被认为对口腔健康以及口腔细菌的生理学和生态学具有重大影响。使用连续恒化器培养，检查了唾液链球菌57.I中的脲酶活性作为生长pH、碳水化合物利用率和生长速率的函数。 S 的一部分。使用编码已知脲酶高度保守序列的简并引物，通过聚合酶链式反应 (PCR) 扩增唾液杆菌 ureC 基因。测定PCR产物的核苷酸序列，并用于比较不同生长条件下脲酶基因表达水平。数据表明脲酶在低pH值下高度表达，并且表达也受到葡萄糖利用率和生长速率的调节。差异表达至少部分地在转录水平上受到控制。
Alkali production from urea by bacterial ureases in the oral cavity is thought to have a major impact on oral health and on the physiology and ecology of oral bacteria. Using continuous chemostat culture, urease activity inStreptococcus salivarius57.I was examined as a function of growth pH, carbohydrate availability and growth rate. A portion of theS. salivarius ureCgene was amplified by polymerase chain reactions (PCRs) using degenerate primers encoding highly conserved sequences from known ureases. The nucleotide sequence of the PCR product was determined, and was used to compare the level of urease gene expression under different growth conditions. The data indicated that urease was highly expressed at low pH, and expression was also modulated by glucose availability and growth rate. Differential expression was controlled, at least in part, at the transcriptional level.