Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45
Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45
复制标题
DOI：
10.1016/s1097-2765(02)00456-2
复制
发表时间：
2002-02-01
期刊：
影响因子：
16
通讯作者：
Walter, JC
中科院分区：
文献类型：
--
作者：
Wohlschlegel, JA;Dhar, SK;Walter, JC
目前的模型表明，复制起始因子Mcm 10是必要的关联Mcm 2 -7与复制起点，以产生复制前复合物（前RC）。在这里，我们报告说，非洲爪蟾Mcm 10（XMcm 10）是不需要的XMcm 2 -7的起源绑定。相反，XMcm 10在DNA复制开始时的染色质结合需要染色质结合的XMcm 2 -7，并且它独立于Cdk 2和Cdc 7。在不存在XMcm 10的情况下，XCdc 45结合、XRPA结合和起始依赖性质粒超螺旋被阻断。因此，XMcm 10在预RC组装之后和原点展开之前执行其功能。作为已知的最早的前RC激活步骤之一，XMcm 10的染色质结合是通过细胞周期检查点调节的有吸引力的靶点。
Current models suggest that the replication initiation factor Mcm10 is required for association of Mcm2-7 with origins of replication to generate the prereplicative complex (pre-RC). Here we report that Xenopus Mcm10 (XMcm10) is not required for origin binding of XMcm2-7. Instead, the chromatin binding of XMcm10 at the onset of DNA replication requires chromatin bound XMcm2-7, and it is independent of Cdk2 and Cdc7. In the absence of XMcm10, XCdc45 binding, XRPA binding, and initiation-dependent plasmid supercoiling are blocked. Therefore, XMcm10 performs its function after pre-RC assembly and before origin unwinding. As one of the earliest known pre-RC activation steps, chromatin binding of XMcm10 is an attractive target for regulation by cell cycle checkpoints.