DETECTION OF HYDROGEN-PEROXIDE AND HYDROXYL RADICALS IN MURINE SKIN FIBROBLASTS UNDER UVB IRRADIATION
DETECTION OF HYDROGEN-PEROXIDE AND HYDROXYL RADICALS IN MURINE SKIN FIBROBLASTS UNDER UVB IRRADIATION
复制标题
DOI：
10.1006/bbrc.1995.1067
复制
发表时间：
1995-01-17
影响因子：
3.1
通讯作者：
SAKURAI, H
中科院分区：
文献类型：
--
作者：
MASAKI, H;ATSUMI, T;SAKURAI, H
本研究的目的是鉴定UVB照射下小鼠皮肤成纤维细胞产生的活性氧种类。当成纤维细胞暴露于紫外线（UVA+UVB）下时，以DMPO作为自旋捕获剂，用esr -自旋捕获法检测羟基自由基。添加典型的羟基自由基清除剂DMSO可防止UVB照射引起的细胞损伤。过氧化氢酶抑制剂3-氨基- 1h -1,2,4-三唑处理的成纤维细胞对UVB辐射的敏感性高于未处理的细胞，但添加DMSO后敏感性有所提高。为了寻找UVB照射下成纤维细胞中羟基自由基的来源，用二氢霍达明123测定H2O2，发现UVB照射下H2O2的形成增加。这些结果表明，uvb照射下的皮肤成纤维细胞产生H2O2，随后形成羟基自由基，可能来源于fenton样反应（H2O2 + Fe2+—>）。OH + OH- + Fe3+)，从而破坏细胞。(C) 1995学术出版社，Inc。
The purpose of this study was to identify the active oxygen species generated in murine skin fibroblasts under UVB irradiation. When fibroblasts were exposed to UV light (UVA+UVB), hydroxyl radicals were detectable by ESR-spin trapping using DMPO as a spin trapping agent. Cell damage induced by UVB irradiation was prevented by addition of DMSO, a typical hydroxyl radical scavenger. Fibroblasts treated with a catalase inhibitor 3-amino-1H-1,2,4-triazole were more sensitive to UVB irradiation than untreated-cells, but the sensitivity was improved by addition of DMSO. To find the origin of the hydroxyl radicals in fibroblasts during UVB irradiation, H2O2 was determined by dihydrorhodamine 123 and it was found that the formation of H2O2 increased under UVB irradiation. These results suggest that UVB-irradiated skin fibroblasts produce H2O2 and were followed by the formation of hydroxyl radicals, probably derived from the Fenton-like reaction (H2O2 + Fe2+-->.OH + OH- + Fe3+), which in turn damages the cells. (C) 1995 Academic Press, Inc.