Use of ionic liquid in fungal taxonomic study of ultrastructure of basidiospore ornamentation
Use of ionic liquid in fungal taxonomic study of ultrastructure of basidiospore ornamentation
复制标题
DOI：
10.1007/s11557-011-0794-6
复制
发表时间：
2012-02-01
影响因子：
2.4
通讯作者：
Kuwabata, Susumu
中科院分区：
文献类型：
--
作者：
Yanaga, Konomi;Maekawa, Nitaro;Kuwabata, Susumu
离子液体（IL）是一种即使在室温下也保持熔融状态的盐。由于离子液体即使在真空条件下也不会蒸发，并表现出高离子电导率，因此可用于扫描电子显微镜 (SEM) 研究。担孢子装饰的超微结构特征被认为对于真菌类群的划分非常重要。在本研究中，我们对经过 IL 处理的担孢子进行了 SEM 观察，并与使用脱水、干燥和铂 (Pt) 涂层的传统制备方法相比，评估了该方法的有效性。使用常规方法，鳃组织中损失了相当数量的担孢子；然而，使用IL方法，担孢子的减少量极小。在 Pt 包被的担孢子和 IL 处理的担孢子之间没有发现超微结构形态或担孢子大小的显着差异。铂涂覆的担孢子的 SEM 图像往往比 IL 处理的担孢子具有更高的对比度。在涂有 Pt 的担孢子上观察到带电效应，特别是在装饰物的尖端，而在 IL 处理的担子孢子上则没有出现这种效应。此外，在 Pt 包被的担孢子中观察到小皱纹，但在 IL 处理的担孢子中没有观察到。这些结果表明 IL 方法对于真菌分类学研究很有用。
An ionic liquid (IL) is a kind of salt that stays in a molten state even at room temperature. Since ILs do not vaporize even under vacuum conditions and show high ionic conductivity, they can be used in scanning electron microscopy (SEM) studies. The ultrastructural features of basidiospore ornamentation are considered to be important in the delimitation of taxa for fungi. In the present study, we carried out SEM observations on basidiospores that were subjected to an IL treatment, and evaluated the usefulness of this method in comparison with a conventional preparation method in which dehydrating, drying and platinum (Pt) coating were used. Using the conventional method, a considerable number of basidiospores was lost from the gill tissues; however, using the IL method, the decrease in basidiospores was extremely small. No significant differences in ultrastructural morphology or basidiospore size were found between Pt-coated basidiospores and IL-treated ones. SEM images of Pt-coated basidiospores tended to have higher contrast than those of IL-treated ones. Charging effects were observed with Pt-coated basidiospores, especially at the tips of the ornaments, whereas no such effects occurred for the IL-treated ones. In addition, small crinkles were observed in the Pt-coated basidiospores, but not in the IL-treated ones. These results suggest that the IL method is useful for fungal taxonomic studies.